Abstract
In the neuroendocrine pancreatic β-cell, elevations in intracellular Ca2+ lead to insulin secretion and the initiation of gene transcription. However, the relationship between cytosolic and nuclear Ca2+ in these cells is unknown. The Ca2+ permeability of the nuclear membrane would therefore determine if Ca2+ could play a direct role in Ca2+-dependent nuclear processes. Using confocal fluorescence microscopy with the ratiometric Ca2+ indicator indo-1 and carefully correcting for compartmentalized indicator, we now demonstrate that there is no difference between the nuclear Ca2+ concentration and the cytosolic Ca2+ concentration ([Ca2+](c)) in the resting β-cell. Slow Ca2+ oscillations induced by glucose, fast oscillations induced by glucagon-like peptide-1 and responses to potassium and carbachol all indicate that changes in cytosolic Ca2+ are reflected within the nucleus. We conclude that there are no restrictions on Ca2+ entry into the nucleus of the pancreatic β-cell subsequent to increases in [Ca2+](c). This implies that any signal involved in increasing [Ca2+](c), and thereby insulin release, may also promote nuclear Ca2+-induced gene transcription.
| Original language | English |
|---|---|
| Pages (from-to) | 771-778 |
| Number of pages | 8 |
| Journal | Biochemical Journal |
| Volume | 325 |
| Issue number | 3 |
| State | Published - Aug 1 1997 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
Cite this
Parallel changes in nuclear and cytosolic calcium in mouse pancreatic β-cells. / Brown, Graham R.; Köhler, Martin; Berggren, Per Olof.
In: Biochemical Journal, Vol. 325, No. 3, 01.08.1997, p. 771-778.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Parallel changes in nuclear and cytosolic calcium in mouse pancreatic β-cells
AU - Brown, Graham R.
AU - Köhler, Martin
AU - Berggren, Per Olof
PY - 1997/8/1
Y1 - 1997/8/1
N2 - In the neuroendocrine pancreatic β-cell, elevations in intracellular Ca2+ lead to insulin secretion and the initiation of gene transcription. However, the relationship between cytosolic and nuclear Ca2+ in these cells is unknown. The Ca2+ permeability of the nuclear membrane would therefore determine if Ca2+ could play a direct role in Ca2+-dependent nuclear processes. Using confocal fluorescence microscopy with the ratiometric Ca2+ indicator indo-1 and carefully correcting for compartmentalized indicator, we now demonstrate that there is no difference between the nuclear Ca2+ concentration and the cytosolic Ca2+ concentration ([Ca2+](c)) in the resting β-cell. Slow Ca2+ oscillations induced by glucose, fast oscillations induced by glucagon-like peptide-1 and responses to potassium and carbachol all indicate that changes in cytosolic Ca2+ are reflected within the nucleus. We conclude that there are no restrictions on Ca2+ entry into the nucleus of the pancreatic β-cell subsequent to increases in [Ca2+](c). This implies that any signal involved in increasing [Ca2+](c), and thereby insulin release, may also promote nuclear Ca2+-induced gene transcription.
AB - In the neuroendocrine pancreatic β-cell, elevations in intracellular Ca2+ lead to insulin secretion and the initiation of gene transcription. However, the relationship between cytosolic and nuclear Ca2+ in these cells is unknown. The Ca2+ permeability of the nuclear membrane would therefore determine if Ca2+ could play a direct role in Ca2+-dependent nuclear processes. Using confocal fluorescence microscopy with the ratiometric Ca2+ indicator indo-1 and carefully correcting for compartmentalized indicator, we now demonstrate that there is no difference between the nuclear Ca2+ concentration and the cytosolic Ca2+ concentration ([Ca2+](c)) in the resting β-cell. Slow Ca2+ oscillations induced by glucose, fast oscillations induced by glucagon-like peptide-1 and responses to potassium and carbachol all indicate that changes in cytosolic Ca2+ are reflected within the nucleus. We conclude that there are no restrictions on Ca2+ entry into the nucleus of the pancreatic β-cell subsequent to increases in [Ca2+](c). This implies that any signal involved in increasing [Ca2+](c), and thereby insulin release, may also promote nuclear Ca2+-induced gene transcription.
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UR - http://www.scopus.com/inward/citedby.url?scp=0030755087&partnerID=8YFLogxK
M3 - Article
C2 - 9271099
AN - SCOPUS:0030755087
VL - 325
SP - 771
EP - 778
JO - Biochemical Journal
JF - Biochemical Journal
SN - 0264-6021
IS - 3
ER -