Parallel changes in nuclear and cytosolic calcium in mouse pancreatic β-cells

Graham R. Brown, Martin Köhler, Per Olof Berggren

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

In the neuroendocrine pancreatic β-cell, elevations in intracellular Ca2+ lead to insulin secretion and the initiation of gene transcription. However, the relationship between cytosolic and nuclear Ca2+ in these cells is unknown. The Ca2+ permeability of the nuclear membrane would therefore determine if Ca2+ could play a direct role in Ca2+-dependent nuclear processes. Using confocal fluorescence microscopy with the ratiometric Ca2+ indicator indo-1 and carefully correcting for compartmentalized indicator, we now demonstrate that there is no difference between the nuclear Ca2+ concentration and the cytosolic Ca2+ concentration ([Ca2+](c)) in the resting β-cell. Slow Ca2+ oscillations induced by glucose, fast oscillations induced by glucagon-like peptide-1 and responses to potassium and carbachol all indicate that changes in cytosolic Ca2+ are reflected within the nucleus. We conclude that there are no restrictions on Ca2+ entry into the nucleus of the pancreatic β-cell subsequent to increases in [Ca2+](c). This implies that any signal involved in increasing [Ca2+](c), and thereby insulin release, may also promote nuclear Ca2+-induced gene transcription.

Original languageEnglish
Pages (from-to)771-778
Number of pages8
JournalBiochemical Journal
Volume325
Issue number3
StatePublished - Aug 1 1997
Externally publishedYes

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Transcription
Genes
Insulin
Calcium
Neuroendocrine Cells
Glucagon-Like Peptide 1
Confocal microscopy
Fluorescence microscopy
Nuclear Envelope
Carbachol
Cell Nucleus
Fluorescence Microscopy
Confocal Microscopy
Permeability
Potassium
Membranes
Glucose
indo-1

ASJC Scopus subject areas

  • Biochemistry

Cite this

Brown, G. R., Köhler, M., & Berggren, P. O. (1997). Parallel changes in nuclear and cytosolic calcium in mouse pancreatic β-cells. Biochemical Journal, 325(3), 771-778.

Parallel changes in nuclear and cytosolic calcium in mouse pancreatic β-cells. / Brown, Graham R.; Köhler, Martin; Berggren, Per Olof.

In: Biochemical Journal, Vol. 325, No. 3, 01.08.1997, p. 771-778.

Research output: Contribution to journalArticle

Brown, GR, Köhler, M & Berggren, PO 1997, 'Parallel changes in nuclear and cytosolic calcium in mouse pancreatic β-cells', Biochemical Journal, vol. 325, no. 3, pp. 771-778.
Brown, Graham R. ; Köhler, Martin ; Berggren, Per Olof. / Parallel changes in nuclear and cytosolic calcium in mouse pancreatic β-cells. In: Biochemical Journal. 1997 ; Vol. 325, No. 3. pp. 771-778.
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