Pannexin channels are not gap junction hemichannels

Gina E. Sosinsky, Daniela Boassa, Rolf Dermietzel, Heather S. Duffy, Dale W. Laird, Brian A. MacVicar, Christian C. Naus, Silvia Penuela, Eliana Scemes, David C. Spray, Roger J. Thompson, Hong Bo Zhao, Gerhard Dahl

Research output: Contribution to journalArticle

206 Citations (Scopus)

Abstract

Pannexins, a class of membrane channels, bear significant sequence homology with the invertebrate gap junction proteins, innexins, and more distant similarities in their membrane topologies and pharmacological sensitivities with the gap junction proteins, connexins. However, the functional role for the pannexin oligomers or pannexons, is different from connexin oligomers, the connexons. Many pannexin publications have used the term " hemichannels" to describe pannexin oligomers while others use the term "channels" instead. This has led to confusion within the literature about the function of pannexins that promotes the idea that pannexons serve as gap junction hemichannels and thus, have an assembly and functional state as gap junctional intercellular channels. Here, we present the case that unlike the connexin gap junction intercellular channels, so far, pannexin oligomers have repeatedly been shown to be channels that are functional in single membranes, but not as intercellular channels in appositional membranes. Hence, they should be referred to as channels and not hemichannels. Thus, we advocate that in the absence of firm evidence that pannexins form gap junctions, the use of the term "hemichannel" be discontinued within the pannexin literature.

Original languageEnglish
JournalChannels
Volume5
Issue number3
StatePublished - May 1 2011

Fingerprint

Connexins
Gap Junctions
Oligomers
Membranes
Invertebrates
Sequence Homology
Ion Channels
Publications
Topology
Pharmacology

Keywords

  • ATP signaling
  • Connexin
  • Connexon
  • Innexins
  • Intercellular communication
  • Pannexin
  • Pannexon
  • Purinergic receptors

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

Cite this

Sosinsky, G. E., Boassa, D., Dermietzel, R., Duffy, H. S., Laird, D. W., MacVicar, B. A., ... Dahl, G. (2011). Pannexin channels are not gap junction hemichannels. Channels, 5(3).

Pannexin channels are not gap junction hemichannels. / Sosinsky, Gina E.; Boassa, Daniela; Dermietzel, Rolf; Duffy, Heather S.; Laird, Dale W.; MacVicar, Brian A.; Naus, Christian C.; Penuela, Silvia; Scemes, Eliana; Spray, David C.; Thompson, Roger J.; Zhao, Hong Bo; Dahl, Gerhard.

In: Channels, Vol. 5, No. 3, 01.05.2011.

Research output: Contribution to journalArticle

Sosinsky, GE, Boassa, D, Dermietzel, R, Duffy, HS, Laird, DW, MacVicar, BA, Naus, CC, Penuela, S, Scemes, E, Spray, DC, Thompson, RJ, Zhao, HB & Dahl, G 2011, 'Pannexin channels are not gap junction hemichannels', Channels, vol. 5, no. 3.
Sosinsky GE, Boassa D, Dermietzel R, Duffy HS, Laird DW, MacVicar BA et al. Pannexin channels are not gap junction hemichannels. Channels. 2011 May 1;5(3).
Sosinsky, Gina E. ; Boassa, Daniela ; Dermietzel, Rolf ; Duffy, Heather S. ; Laird, Dale W. ; MacVicar, Brian A. ; Naus, Christian C. ; Penuela, Silvia ; Scemes, Eliana ; Spray, David C. ; Thompson, Roger J. ; Zhao, Hong Bo ; Dahl, Gerhard. / Pannexin channels are not gap junction hemichannels. In: Channels. 2011 ; Vol. 5, No. 3.
@article{2a56428149b944c88290d3c407c0e74c,
title = "Pannexin channels are not gap junction hemichannels",
abstract = "Pannexins, a class of membrane channels, bear significant sequence homology with the invertebrate gap junction proteins, innexins, and more distant similarities in their membrane topologies and pharmacological sensitivities with the gap junction proteins, connexins. However, the functional role for the pannexin oligomers or pannexons, is different from connexin oligomers, the connexons. Many pannexin publications have used the term {"} hemichannels{"} to describe pannexin oligomers while others use the term {"}channels{"} instead. This has led to confusion within the literature about the function of pannexins that promotes the idea that pannexons serve as gap junction hemichannels and thus, have an assembly and functional state as gap junctional intercellular channels. Here, we present the case that unlike the connexin gap junction intercellular channels, so far, pannexin oligomers have repeatedly been shown to be channels that are functional in single membranes, but not as intercellular channels in appositional membranes. Hence, they should be referred to as channels and not hemichannels. Thus, we advocate that in the absence of firm evidence that pannexins form gap junctions, the use of the term {"}hemichannel{"} be discontinued within the pannexin literature.",
keywords = "ATP signaling, Connexin, Connexon, Innexins, Intercellular communication, Pannexin, Pannexon, Purinergic receptors",
author = "Sosinsky, {Gina E.} and Daniela Boassa and Rolf Dermietzel and Duffy, {Heather S.} and Laird, {Dale W.} and MacVicar, {Brian A.} and Naus, {Christian C.} and Silvia Penuela and Eliana Scemes and Spray, {David C.} and Thompson, {Roger J.} and Zhao, {Hong Bo} and Gerhard Dahl",
year = "2011",
month = "5",
day = "1",
language = "English",
volume = "5",
journal = "Channels",
issn = "1933-6950",
publisher = "Landes Bioscience",
number = "3",

}

TY - JOUR

T1 - Pannexin channels are not gap junction hemichannels

AU - Sosinsky, Gina E.

AU - Boassa, Daniela

AU - Dermietzel, Rolf

AU - Duffy, Heather S.

AU - Laird, Dale W.

AU - MacVicar, Brian A.

AU - Naus, Christian C.

AU - Penuela, Silvia

AU - Scemes, Eliana

AU - Spray, David C.

AU - Thompson, Roger J.

AU - Zhao, Hong Bo

AU - Dahl, Gerhard

PY - 2011/5/1

Y1 - 2011/5/1

N2 - Pannexins, a class of membrane channels, bear significant sequence homology with the invertebrate gap junction proteins, innexins, and more distant similarities in their membrane topologies and pharmacological sensitivities with the gap junction proteins, connexins. However, the functional role for the pannexin oligomers or pannexons, is different from connexin oligomers, the connexons. Many pannexin publications have used the term " hemichannels" to describe pannexin oligomers while others use the term "channels" instead. This has led to confusion within the literature about the function of pannexins that promotes the idea that pannexons serve as gap junction hemichannels and thus, have an assembly and functional state as gap junctional intercellular channels. Here, we present the case that unlike the connexin gap junction intercellular channels, so far, pannexin oligomers have repeatedly been shown to be channels that are functional in single membranes, but not as intercellular channels in appositional membranes. Hence, they should be referred to as channels and not hemichannels. Thus, we advocate that in the absence of firm evidence that pannexins form gap junctions, the use of the term "hemichannel" be discontinued within the pannexin literature.

AB - Pannexins, a class of membrane channels, bear significant sequence homology with the invertebrate gap junction proteins, innexins, and more distant similarities in their membrane topologies and pharmacological sensitivities with the gap junction proteins, connexins. However, the functional role for the pannexin oligomers or pannexons, is different from connexin oligomers, the connexons. Many pannexin publications have used the term " hemichannels" to describe pannexin oligomers while others use the term "channels" instead. This has led to confusion within the literature about the function of pannexins that promotes the idea that pannexons serve as gap junction hemichannels and thus, have an assembly and functional state as gap junctional intercellular channels. Here, we present the case that unlike the connexin gap junction intercellular channels, so far, pannexin oligomers have repeatedly been shown to be channels that are functional in single membranes, but not as intercellular channels in appositional membranes. Hence, they should be referred to as channels and not hemichannels. Thus, we advocate that in the absence of firm evidence that pannexins form gap junctions, the use of the term "hemichannel" be discontinued within the pannexin literature.

KW - ATP signaling

KW - Connexin

KW - Connexon

KW - Innexins

KW - Intercellular communication

KW - Pannexin

KW - Pannexon

KW - Purinergic receptors

UR - http://www.scopus.com/inward/record.url?scp=79955717821&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79955717821&partnerID=8YFLogxK

M3 - Article

C2 - 21532340

AN - SCOPUS:79955717821

VL - 5

JO - Channels

JF - Channels

SN - 1933-6950

IS - 3

ER -