Pannexin 1: The molecular substrate of astrocyte "hemichannels"

Rodolfo Iglesias, Gerhard Dahl, Feng Qiu, David C. Spray, Eliana Scemes

Research output: Contribution to journalArticle

274 Scopus citations

Abstract

Purinergic signaling plays distinct and important roles in the CNS, including the transmission of calcium signals between astrocytes. Gap junction hemichannels are among the mechanisms proposed by which astrocytes might release ATP; however, whether the gap junction protein connexin43 (Cx43) forms these "hemichannels" remains controversial. Recently, a new group of proteins, the pannexins, have been shown to form nonselective, high-conductance plasmalemmal channels permeable to ATP, thereby offering an alternative for the hemichannel protein. Here, we provide strong evidence that, in cultured astrocytes, pannexin1 (Panx1) but not Cx43 forms hemichannels. Electrophysiological and fluorescence microscope recordings performed in wild-type and Cx43-null astrocytes did not reveal any differences in hemichannel activity, which was mostly eliminated by treating Cx43-null astrocytes with Panx1-short interfering RNA [Panx1-knockdown (Panx1-KD)]. Moreover, quantification of the amount of ATP released from wild-type, Cx43-null, and Panx1-KD astrocytes indicates that downregulation of Panx1, but not of Cx43, prevented ATP release from these cells.

Original languageEnglish (US)
Pages (from-to)7092-7097
Number of pages6
JournalJournal of Neuroscience
Volume29
Issue number21
DOIs
StatePublished - May 27 2009

ASJC Scopus subject areas

  • Neuroscience(all)

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