Pannexin 1 facilitates arterial relaxation via an endothelium-derived hyperpolarization mechanism

Dina Gaynullina, Valery I. Shestopalov, Yury Panchin, Olga S. Tarasova

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Pannexin 1 (Panx1) is involved in endothelium-dependent vasodilation in large arteries, but the exact mechanistic role remains poorly understood. We hypothesized that Panx1 facilitates large vessel relaxations regulating endothelium-derived hyperpolarization (EDH)-like mechanisms. The EDH-like component of acetylcholine-induced relaxation of saphenous arteries studied in isometric myograph after inhibition of NO-synthase and cyclooxygenase was significantly impaired in mice with genetically ablated Panx1 (KO) relative to that in the wild type (WT) mice. Application of P1-receptor antagonist and apyrase significantly reduced this component in WT, but not in KO mice, indicating participation of ATP released via Panx1 in the EDH-like relaxation.

Original languageEnglish (US)
Pages (from-to)1164-1170
Number of pages7
JournalFEBS letters
Volume589
Issue number10
DOIs
StatePublished - Apr 28 2015

Keywords

  • ATP
  • EDHF
  • Endothelium
  • Endothelium-derived hyperpolarization
  • Knockout mice
  • Pannexin 1
  • Pannexins
  • Saphenous artery

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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