Pancreatic islet transplantation: utility of ductular obstruction and exocrine atrophy model?

A. Verma, A. Dinda, C. Sarkar, R. Fotedar, I. K. Dhawan, L. K. Sharma, K. Khetarpal, A. Fotedar, S. Srikanta, N. Kochupillai

Research output: Contribution to journalArticle

Abstract

Introduction of 'silent' exocrine atrophy (and endocrine 'enrichment') in pancreatic grafts following ductular blockade may have a role in human diabetes by circumventing currently elusive islet isolation/purification protocols. To explore this potential, pancreatic isografts were performed in 12 pairs of inbred Wistar NIN rats. Donor pancreatectomy was performed after distal clamping and canulation of common bile duct and injection of 0.5 ml. polyacrylamide gel (blocked n = 7) or normal saline (un-blocked n = 5) respectively. One to 2 m.m. fragments of the resulting mildly distended pancreases were transplanted in to 2 sites (renal capsule and iliac fossa subcutaneously) of cach recipient. Post-operative biopsies of the transplanted grafts (unilateral nephrectomy and iliac fossa biopsies) revealed macroscopic and microscopic evidence of necrotizing pancreatitis in both the groups at both the sites (histiocytic and giant cell infiltration, fat necrosis and focal calcification with destruction of exocrine and endocrine cells) as early as 1 and 3 weeks. Possible detrimental factors include: volume and pressure of ductal injection, graft sites (confined spaces), post-operative wound infection and bio-compatibility of the material used for ductular blockade.

Original languageEnglish (US)
Pages (from-to)148-151
Number of pages4
JournalTropical gastroenterology : official journal of the Digestive Diseases Foundation
Volume11
Issue number3
StatePublished - Jan 1 1990
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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    Verma, A., Dinda, A., Sarkar, C., Fotedar, R., Dhawan, I. K., Sharma, L. K., Khetarpal, K., Fotedar, A., Srikanta, S., & Kochupillai, N. (1990). Pancreatic islet transplantation: utility of ductular obstruction and exocrine atrophy model? Tropical gastroenterology : official journal of the Digestive Diseases Foundation, 11(3), 148-151.