Pancreatic islet function in a transgenic mouse expressing fluorescent protein

Daniel Nyqvist, Göran Mattsson, Martin Köhler, Varda Lev-Ram, Arne Andersson, Per Ola Carlsson, Astrid Nordin, Per Olof Berggren, Leif Jansson

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Pancreatic islet function and glucose homeostasis have been characterized in the transgenic YC-3.0 mouse, which expresses the yellow chameleon 3.0 (YC-3.0) protein under the control of the β-actin and the cytomegalovirus promoters. Fluorescence from the enhanced yellow fluorescent protein (EYFP), one part of the yellow chameleon protein, was used as a reporter of transgene expression. EYFP was expressed in different quantities throughout most cell types, including islet endocrine and stromal cells. No adverse effects of the transgene on animal health, growth or fertility were observed. Likewise, in vivo glucose homeostasis, mean arterial blood pressure and regional blood flow values were normal. Furthermore, the transgenic YC-3.0 mouse had a normal β-cell volume and mass as well as glucose-stimulated insulin release in vitro, compared with the C57BL/6 control mouse. Isolated islets from YC-3.0 animals continuously expressed the transgene and reversed hyperglycemia when transplanted under the renal capsule of alloxan-diabetic nude mice. We conclude that isolated pancreatic islets from YC-3.0 animals implanted into recipients without any EYFP expression, constitute a novel and versatile model for studies of islet engraftment.

Original languageEnglish (US)
Pages (from-to)333-341
Number of pages9
JournalJournal of Endocrinology
Volume186
Issue number2
DOIs
StatePublished - Aug 1 2005

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

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    Nyqvist, D., Mattsson, G., Köhler, M., Lev-Ram, V., Andersson, A., Carlsson, P. O., Nordin, A., Berggren, P. O., & Jansson, L. (2005). Pancreatic islet function in a transgenic mouse expressing fluorescent protein. Journal of Endocrinology, 186(2), 333-341. https://doi.org/10.1677/joe.1.06204