Pancreatic duct obstruction in rabbits causes digestive zymogen and lysosomal enzyme colocalization

Ashok Saluja, M. Saluja, A. Villa, U. Leli, P. Rutledge, J. Meldolesi, M. Steer

Research output: Contribution to journalArticle

125 Citations (Scopus)

Abstract

The pancreatic duct of anesthetized rabbits was cannulated and, in some animals, flow of pancreatic exocrine secretions was blocked by raising the cannula to a vertical position. Blockage for 3-7 h caused a rapid and significant rise in serum amylase activity and an increase in amylase activity within the pancreas. The concentration of lysosomal enzymes in the pancreas was not altered but they became redistributed among subcellular fractions and, as a result, an increased amount was recovered in the 1,000-g, 15-min pellet, which was enriched in zymogen granules. Immunofluorescence studies indicated that lysosomal enzymes become localized within organelles which, in size and distribution, resemble zymogen granules. They also contain digestive enzyme zymogens. Blockage of pancreatic secretions also caused lysosomal enzyme-containing organelles to become more fragile and subject to in vitro rupture. These changes noted after short-term pancreatic duct obstruction are remarkably similar to those previously noted to occur during the early stages of diet and secretagogue-induced experimental pancreatitis, observations that have suggested that colocalization of digestive enzyme zymogens and lysosomal hydrolases might result in intracellular digestive enzyme activation and be an important early event in the evolution of those forms of experimental acute pancreatitis.

Original languageEnglish (US)
Pages (from-to)1260-1266
Number of pages7
JournalJournal of Clinical Investigation
Volume84
Issue number4
DOIs
StatePublished - Jan 1 1989
Externally publishedYes

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Enzyme Precursors
Pancreatic Ducts
Rabbits
Enzymes
Secretory Vesicles
Amylases
Pancreatitis
Organelles
Pancreas
Enzyme Activation
Subcellular Fractions
Hydrolases
Fluorescent Antibody Technique
Rupture
Diet
Serum

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Pancreatic duct obstruction in rabbits causes digestive zymogen and lysosomal enzyme colocalization. / Saluja, Ashok; Saluja, M.; Villa, A.; Leli, U.; Rutledge, P.; Meldolesi, J.; Steer, M.

In: Journal of Clinical Investigation, Vol. 84, No. 4, 01.01.1989, p. 1260-1266.

Research output: Contribution to journalArticle

Saluja, Ashok ; Saluja, M. ; Villa, A. ; Leli, U. ; Rutledge, P. ; Meldolesi, J. ; Steer, M. / Pancreatic duct obstruction in rabbits causes digestive zymogen and lysosomal enzyme colocalization. In: Journal of Clinical Investigation. 1989 ; Vol. 84, No. 4. pp. 1260-1266.
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