Pan-neuronal imaging in roaming Caenorhabditis elegans

Vivek Venkatachalam, Ni Ji, Xian Wang, Christopher Clark, James Kameron Mitchell, Mason Klein, Christopher J. Tabone, Jeremy Florman, Hongfei Ji, Joel Greenwood, Andrew D. Chisholm, Jagan Srinivasan, Mark Alkema, Mei Zhen, Aravinthan D T Samuel

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

We present an imaging system for pan-neuronal recording in crawling Caenorhabditis elegans. A spinning disk confocal microscope, modified for automated tracking of the C. elegans head ganglia, simultaneously records the activity and position of ∼80 neurons that coexpress cytoplasmic calcium indicator GCaMP6s and nuclear localized red fluorescent protein at 10 volumes per second. We developed a behavioral analysis algorithm that maps the movements of the head ganglia to the animal's posture and locomotion. Image registration and analysis software automatically assigns an index to each nucleus and calculates the corresponding calcium signal. Neurons with highly stereotyped positions can be associated with unique indexes and subsequently identified using an atlas of the worm nervous system. To test our system, we analyzed the brainwide activity patterns of moving worms subjected to thermosensory inputs. We demonstrate that our setup is able to uncover representations of sensory input and motor output of individual neurons from brainwide dynamics. Our imaging setup and analysis pipeline should facilitate mapping circuits for sensory to motor transformation in transparent behaving animals such as C. elegans and Drosophila larva.

Original languageEnglish (US)
Pages (from-to)E1082-E1088
JournalProceedings of the National Academy of Sciences of the United States of America
Volume113
Issue number8
DOIs
StatePublished - Feb 23 2016
Externally publishedYes

Fingerprint

Caenorhabditis elegans
Neurons
Ganglia
Calcium
Head Movements
Atlases
Locomotion
Posture
Nervous System
Drosophila
Larva
Software
Head

Keywords

  • C. elegans
  • Calcium imaging
  • Drosophila
  • Thermotaxis

ASJC Scopus subject areas

  • General

Cite this

Pan-neuronal imaging in roaming Caenorhabditis elegans. / Venkatachalam, Vivek; Ji, Ni; Wang, Xian; Clark, Christopher; Mitchell, James Kameron; Klein, Mason; Tabone, Christopher J.; Florman, Jeremy; Ji, Hongfei; Greenwood, Joel; Chisholm, Andrew D.; Srinivasan, Jagan; Alkema, Mark; Zhen, Mei; Samuel, Aravinthan D T.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 113, No. 8, 23.02.2016, p. E1082-E1088.

Research output: Contribution to journalArticle

Venkatachalam, V, Ji, N, Wang, X, Clark, C, Mitchell, JK, Klein, M, Tabone, CJ, Florman, J, Ji, H, Greenwood, J, Chisholm, AD, Srinivasan, J, Alkema, M, Zhen, M & Samuel, ADT 2016, 'Pan-neuronal imaging in roaming Caenorhabditis elegans', Proceedings of the National Academy of Sciences of the United States of America, vol. 113, no. 8, pp. E1082-E1088. https://doi.org/10.1073/pnas.1507109113
Venkatachalam, Vivek ; Ji, Ni ; Wang, Xian ; Clark, Christopher ; Mitchell, James Kameron ; Klein, Mason ; Tabone, Christopher J. ; Florman, Jeremy ; Ji, Hongfei ; Greenwood, Joel ; Chisholm, Andrew D. ; Srinivasan, Jagan ; Alkema, Mark ; Zhen, Mei ; Samuel, Aravinthan D T. / Pan-neuronal imaging in roaming Caenorhabditis elegans. In: Proceedings of the National Academy of Sciences of the United States of America. 2016 ; Vol. 113, No. 8. pp. E1082-E1088.
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