Painful neuropathy alters the effect of gabapentin on sensory neuron excitability in rats

Akifumi Kanai, Konstantinos D. Sarantopoulos, J. B. McCallum, Q. Hogan

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Background: Pain following peripheral nerve injury is associated with increased excitability of sensory neurons. Gabapentin (GBP), a novel anticonvulsant with an uncertain mechanism of action, is an effective treatment for neuropathic pain. We therefore investigated the effect of GBP on dorsal root ganglion (DRG) neurons from normal rats and those with painful peripheral nerve injury. Methods: Dorsal root ganglions were excised from rats with neuropathic pain behaviour following chronic constriction injury (CCI) of the sciatic nerve, and from normal rats. Intercellular recordings were made from myelinated sensory neuron somata using a microelectrode technique from DRGs bathed in artificial CSF with or without GBP (100 μM). Results: Compared with normal neurons, injury decreased the refractory interval (RI) for repeat action potential (AP) generation increased the number of APs during sustained depolarization, and shortened the after hyperpolarization following an AP. In normal neurons, GBP decreased the RI and increased the AP number during sustained depolarization. In an opposite fashion, the result of GBP application to injured neurons was a decreased number of APs during depolarization and no change in RI. In injured neurons only, GBP increased the time-to-peak for AP depolarization. Conclusions: Nerve injury by CCI is associated with increased sensory neuron excitability, associated with a decreased AHP. In normal peripheral sensory neurons, GBP has pro-excitatory effects, whereas GBP decreases excitability in injured neurons, possibly on the basis of altered sodium channel function.

Original languageEnglish
Pages (from-to)507-512
Number of pages6
JournalActa Anaesthesiologica Scandinavica
Volume48
Issue number4
DOIs
StatePublished - Apr 1 2004
Externally publishedYes

Fingerprint

Sensory Receptor Cells
Neurons
Action Potentials
Peripheral Nerve Injuries
Wounds and Injuries
Spinal Ganglia
Neuralgia
Constriction
Painful Neuropathy
gabapentin
Sodium Channels
Diagnosis-Related Groups
Carisoprodol
Microelectrodes
Sciatic Nerve
Anticonvulsants
Pain

Keywords

  • Gabapentin
  • Nerve injury
  • Neuronal excitability
  • Neuropathic pain

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

Cite this

Painful neuropathy alters the effect of gabapentin on sensory neuron excitability in rats. / Kanai, Akifumi; Sarantopoulos, Konstantinos D.; McCallum, J. B.; Hogan, Q.

In: Acta Anaesthesiologica Scandinavica, Vol. 48, No. 4, 01.04.2004, p. 507-512.

Research output: Contribution to journalArticle

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