p6gag of human and simian immunodeficiency viruses is tolerant to small in-frame deletions downstream of the late domain

Cheryl A. Pikora, Christine Wittish, Ronald C. Desrosiers

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Short, unique, in-frame deletions were consistently detected within p6gag sequences obtained over time from three of eight HIV-1-infected long-term nonprogressors (Alexander, L., Weiskopf, E., Greenough, T.C., Gaddis, N.C., Auerbach, M.R., Malim, M.H., O'Brien, S.J., Walker, B.D., Sullivan, J.L., Desrosiers, R.C., 2000. Unusual polymorphisms in Human Immunodeficiency Virus Type 1 associated with nonprogressive infection. J. Virol. 74, 4361-4376). Using PCR mutagenesis, we created 11 mutant forms of SIV239 and 8 mutant forms of HIV-1 NL4-3 with serial 2 amino acid deletions within p6gag downstream of the PTAP late domain. Nine of the 11 SIV239 mutants assembled and released virion particles similar to wild-type, displayed wild-type infectivity, and replicated similar to wild-type SIV239 in cultured cells. Two of the 11 SIV239 mutants, both involving D at position 21, were grossly defective for intracellular gag accumulation and did not replicate detectably in cultured cells. Similar to the 9 SIV239 mutants, 7 of the 8 HIV-1 mutants replicated well in cultured cells. Only the mutant deleted of ES at positions 19 and 20, immediately adjacent to the PTAP sequence, was markedly impaired in its replicative capacity. These results demonstrate an overall high tolerance of SIV and HIV to two amino acid deletions within p6gag downstream of the late domain.

Original languageEnglish (US)
Pages (from-to)479-489
Number of pages11
JournalVirology
Volume346
Issue number2
DOIs
StatePublished - Mar 15 2006
Externally publishedYes

Keywords

  • HIV
  • LTNP
  • SIV
  • p6gag

ASJC Scopus subject areas

  • Virology

Fingerprint Dive into the research topics of 'p6gag of human and simian immunodeficiency viruses is tolerant to small in-frame deletions downstream of the late domain'. Together they form a unique fingerprint.

  • Cite this