Abstract
We have previously shown that jacalin, a CD4+ T cell lectin, induces phosphorylation of intracellular events, moderate levels of interleukin (IL)-2 secretion. We have also shown that in the presence of CD28 costimulation, jacalin induces IL-4 secretion. In the present study, we showed that stimulation of normal CD4+ T cells with jacalin plus CD28 cross-linking (CD28XL) resulted in phosphorylation of signal transducer and activator of transcription (STAT)-6 and expression of Bcl-2 and Bcl-xL, which were inhibited significantly when cells were cultured in the presence of the p38 mitogen-activated protein kinase (MAPK) inhibitor SB203580. We further generated jacalin-induced CD4+ T cell blasts, examined the effects of CD28XL, and observed enhanced up-regulation of p38 and activation of STAT-6, Bcl-2, and Bcl-xL. Engagement of CD28 alone induced a marked degree of phosphorylation of p38 MAPK and IL-4 secretion in memory T cells (jacalin blasts), whereas in naïve T cells, jacalin plus CD28XL was required to induce these molecules. Incubation of cells with p38 inhibitor prior to CD28XL resulted in down-modulation of all these molecules. Further treatment with IL-4 has not reversed this trend. Our studies imply that p38 MAPK may play an important role in induction of these molecules and a putative role in protecting cells from undergoing apoptosis.
| Original language | English |
|---|---|
| Pages (from-to) | 1339-1347 |
| Number of pages | 9 |
| Journal | Journal of Leukocyte Biology |
| Volume | 79 |
| Issue number | 6 |
| DOIs | |
| State | Published - Jun 1 2006 |
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Keywords
- Bcl-2
- Bcl-xL
- STAT-6
ASJC Scopus subject areas
- Cell Biology
Cite this
p38 MAPK plays a role in IL-4 synthesis in jacalin plus CD28-stimulated CD4+ T cells - II. / Tamma, Seetha M Lakshmi; Kun, Wook Chung; Patel, Tejal; Balan, Satya Priya; Pahwa, Savita G.
In: Journal of Leukocyte Biology, Vol. 79, No. 6, 01.06.2006, p. 1339-1347.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - p38 MAPK plays a role in IL-4 synthesis in jacalin plus CD28-stimulated CD4+ T cells - II
AU - Tamma, Seetha M Lakshmi
AU - Kun, Wook Chung
AU - Patel, Tejal
AU - Balan, Satya Priya
AU - Pahwa, Savita G
PY - 2006/6/1
Y1 - 2006/6/1
N2 - We have previously shown that jacalin, a CD4+ T cell lectin, induces phosphorylation of intracellular events, moderate levels of interleukin (IL)-2 secretion. We have also shown that in the presence of CD28 costimulation, jacalin induces IL-4 secretion. In the present study, we showed that stimulation of normal CD4+ T cells with jacalin plus CD28 cross-linking (CD28XL) resulted in phosphorylation of signal transducer and activator of transcription (STAT)-6 and expression of Bcl-2 and Bcl-xL, which were inhibited significantly when cells were cultured in the presence of the p38 mitogen-activated protein kinase (MAPK) inhibitor SB203580. We further generated jacalin-induced CD4+ T cell blasts, examined the effects of CD28XL, and observed enhanced up-regulation of p38 and activation of STAT-6, Bcl-2, and Bcl-xL. Engagement of CD28 alone induced a marked degree of phosphorylation of p38 MAPK and IL-4 secretion in memory T cells (jacalin blasts), whereas in naïve T cells, jacalin plus CD28XL was required to induce these molecules. Incubation of cells with p38 inhibitor prior to CD28XL resulted in down-modulation of all these molecules. Further treatment with IL-4 has not reversed this trend. Our studies imply that p38 MAPK may play an important role in induction of these molecules and a putative role in protecting cells from undergoing apoptosis.
AB - We have previously shown that jacalin, a CD4+ T cell lectin, induces phosphorylation of intracellular events, moderate levels of interleukin (IL)-2 secretion. We have also shown that in the presence of CD28 costimulation, jacalin induces IL-4 secretion. In the present study, we showed that stimulation of normal CD4+ T cells with jacalin plus CD28 cross-linking (CD28XL) resulted in phosphorylation of signal transducer and activator of transcription (STAT)-6 and expression of Bcl-2 and Bcl-xL, which were inhibited significantly when cells were cultured in the presence of the p38 mitogen-activated protein kinase (MAPK) inhibitor SB203580. We further generated jacalin-induced CD4+ T cell blasts, examined the effects of CD28XL, and observed enhanced up-regulation of p38 and activation of STAT-6, Bcl-2, and Bcl-xL. Engagement of CD28 alone induced a marked degree of phosphorylation of p38 MAPK and IL-4 secretion in memory T cells (jacalin blasts), whereas in naïve T cells, jacalin plus CD28XL was required to induce these molecules. Incubation of cells with p38 inhibitor prior to CD28XL resulted in down-modulation of all these molecules. Further treatment with IL-4 has not reversed this trend. Our studies imply that p38 MAPK may play an important role in induction of these molecules and a putative role in protecting cells from undergoing apoptosis.
KW - Bcl-2
KW - Bcl-xL
KW - STAT-6
UR - http://www.scopus.com/inward/record.url?scp=33746891256&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33746891256&partnerID=8YFLogxK
U2 - 10.1189/jlb.0905513
DO - 10.1189/jlb.0905513
M3 - Article
C2 - 16554354
AN - SCOPUS:33746891256
VL - 79
SP - 1339
EP - 1347
JO - Journal of Leukocyte Biology
JF - Journal of Leukocyte Biology
SN - 0741-5400
IS - 6
ER -