p18Ink4c collaborates with Men1 to constrain lung stem cell expansion and suppress non-small-cell lung cancers

Xin-Hai Pei, Feng Bai, Matthew D. Smith, Yue Xiong

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Mutant mice lacking both cyclin-dependent kinase (CDK) inhibitors p18 Ink4c and p21Kip1 develop a tumor spectrum reminiscent of human multiple endocrine neoplasia (MEN) syndromes. To determine how p18 and p27 genetically interact with Men1, the tumor suppressor gene mutated in familial MEN1, we characterized p18-Men1 and p27-Men1 double mutant mice and showed that p18, but not p27, functionally collaborates with Men1 in suppressing lung tumorigenesis. Lung tumors developed in both Men1+/1 and p18 -/-;Men1+/- mice at a high penetrance and contain both neuroendocrine and nonneuroendocrine cells. The remaining wild-type Men1 allele was lost in most lung tumors from Men1+/- mice but was retained in most tumors from p18-/-;Men1+/- mice, showing a functional collaboration between p18 and Men1 in lung tumor suppression. Phosphorylation of Rb protein at both CDK2 and CDK4/CDK6 sites were significantly increased in normal bronchial epithelia and tumor cells derived from p18- /-;Men1+/- mice compared to those from single p18 -/- or Men1+/- mice. Lung tumors developed in p18 -/-;Men1+/- mice were multifocal, more heterogeneous, and highly invasive compared to those developed in either p18-/- or Men1+/- mice. Rronchioalveolar stem cells are expanded in normal and tumorigenic lungs of p18-/- mice and are further expanded in p18 -/-;Men1+/- lung tumors. These results reveal a previously unrecognized function of p18 in lung tumor suppression through collaboration with Men1 to control lung stem cell proliferation.

Original languageEnglish
Pages (from-to)3162-3170
Number of pages9
JournalCancer Research
Volume67
Issue number7
DOIs
StatePublished - Apr 1 2007
Externally publishedYes

Fingerprint

Non-Small Cell Lung Carcinoma
Stem Cells
Lung
Neoplasms
Cyclin-Dependent Kinase Inhibitor p18
Multiple Endocrine Neoplasia
Multiple Endocrine Neoplasia Type 1
Neuroendocrine Cells
Retinoblastoma Protein
Penetrance
Tumor Suppressor Genes
Carcinogenesis
Epithelium
Alleles
Phosphorylation
Cell Proliferation

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

p18Ink4c collaborates with Men1 to constrain lung stem cell expansion and suppress non-small-cell lung cancers. / Pei, Xin-Hai; Bai, Feng; Smith, Matthew D.; Xiong, Yue.

In: Cancer Research, Vol. 67, No. 7, 01.04.2007, p. 3162-3170.

Research output: Contribution to journalArticle

Pei, Xin-Hai ; Bai, Feng ; Smith, Matthew D. ; Xiong, Yue. / p18Ink4c collaborates with Men1 to constrain lung stem cell expansion and suppress non-small-cell lung cancers. In: Cancer Research. 2007 ; Vol. 67, No. 7. pp. 3162-3170.
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abstract = "Mutant mice lacking both cyclin-dependent kinase (CDK) inhibitors p18 Ink4c and p21Kip1 develop a tumor spectrum reminiscent of human multiple endocrine neoplasia (MEN) syndromes. To determine how p18 and p27 genetically interact with Men1, the tumor suppressor gene mutated in familial MEN1, we characterized p18-Men1 and p27-Men1 double mutant mice and showed that p18, but not p27, functionally collaborates with Men1 in suppressing lung tumorigenesis. Lung tumors developed in both Men1+/1 and p18 -/-;Men1+/- mice at a high penetrance and contain both neuroendocrine and nonneuroendocrine cells. The remaining wild-type Men1 allele was lost in most lung tumors from Men1+/- mice but was retained in most tumors from p18-/-;Men1+/- mice, showing a functional collaboration between p18 and Men1 in lung tumor suppression. Phosphorylation of Rb protein at both CDK2 and CDK4/CDK6 sites were significantly increased in normal bronchial epithelia and tumor cells derived from p18- /-;Men1+/- mice compared to those from single p18 -/- or Men1+/- mice. Lung tumors developed in p18 -/-;Men1+/- mice were multifocal, more heterogeneous, and highly invasive compared to those developed in either p18-/- or Men1+/- mice. Rronchioalveolar stem cells are expanded in normal and tumorigenic lungs of p18-/- mice and are further expanded in p18 -/-;Men1+/- lung tumors. These results reveal a previously unrecognized function of p18 in lung tumor suppression through collaboration with Men1 to control lung stem cell proliferation.",
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