P-type proton ATPases are involved in intracellular calcium and proton uptake in the plant parasite Phytomonas francai

Kildare Miranda, Anibal E. Vercesi, Rosana Catisti, Wanderley De Souza, Claudia D Rodrigues, Roberto Docampo

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The use of digitonin to permeabilize the plasma membrane of promastigotes of Phytomonas francai allowed the identification of two non-mitochondrial Ca2+ compartments; one sensitive to ionomycin and vanadate (neutral or alkaline), possibly the endoplasmic reticulum, and another sensitive to the combination of nigericin plus ionomycin (acidic), possibly the acidocalcisomes. A P-type (phospho-intermediate form) Ca2+-ATPase activity was found to be responsible for intracellular Ca2+ transport in these cells, with no evidence of a mitochondrial Ca2+ transport activity. ATP-driven acidification of internal compartments in cell lysates and cells mechanically permeabilized was assayed spectrophotometrically with acridine orange. This activity was inhibited by low concentrations of vanadate and digitonin, was insensitive to bafilomycin A1, and stimulated by Na+ ions. Taken together, our results indicate that P-type ATPases are involved in intracellular Ca2+ and H+ transport in promastigotes of P. francai.

Original languageEnglish
Pages (from-to)55-60
Number of pages6
JournalJournal of Eukaryotic Microbiology
Volume52
Issue number1
DOIs
StatePublished - Feb 1 2005
Externally publishedYes

Fingerprint

Phytomonas
protons
adenosinetriphosphatase
Adenosine Triphosphatases
Protons
Digitonin
Parasites
Ionomycin
Vanadates
digitonin
Calcium
uptake mechanisms
calcium
parasites
promastigotes
Nigericin
Acridine Orange
Calcium-Transporting ATPases
nigericin
Endoplasmic Reticulum

Keywords

  • Calcium ATPase
  • Digitonin
  • Phytomonas
  • Proton ATPase

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Applied Microbiology and Biotechnology
  • Agricultural and Biological Sciences (miscellaneous)
  • Microbiology

Cite this

P-type proton ATPases are involved in intracellular calcium and proton uptake in the plant parasite Phytomonas francai. / Miranda, Kildare; Vercesi, Anibal E.; Catisti, Rosana; De Souza, Wanderley; Rodrigues, Claudia D; Docampo, Roberto.

In: Journal of Eukaryotic Microbiology, Vol. 52, No. 1, 01.02.2005, p. 55-60.

Research output: Contribution to journalArticle

Miranda, Kildare ; Vercesi, Anibal E. ; Catisti, Rosana ; De Souza, Wanderley ; Rodrigues, Claudia D ; Docampo, Roberto. / P-type proton ATPases are involved in intracellular calcium and proton uptake in the plant parasite Phytomonas francai. In: Journal of Eukaryotic Microbiology. 2005 ; Vol. 52, No. 1. pp. 55-60.
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