Oxytocin attenuates atherosclerosis and adipose tissue inflammation in socially isolated ApoE-/- Mice

Daniel A. Nation, Angela Szeto, Armando J Mendez, Larry G. Brooks, Julia Zaias, Edward E. Herderick, Julie Gonzales, Crystal M. Noller, Neil Schneiderman, Philip McCabe

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

OBJECTIVE: To determine the effect of exogenous oxytocin (OT) administration on inflammation and atherosclerosis in socially isolated apoE mice. Hyperlipidemic animals housed in isolated or stressful social environments display more extensive atherosclerosis than those in an affiliative social environment. The neurohypophyseal peptide OT may be involved in both affiliative social behavior and cardiovascular homeostasis, suggesting a role in mediating the benefits of positive social interactions on atherosclerosis. METHODS: A total of 43, 12-week-old, apoE-/- mice were surgically implanted with osmotic minipumps containing OT (n = 23) or vehicle (n = 20). Blood samples were taken at baseline and after 6 weeks and 12 weeks of treatment. After 12 weeks of treatment, animals were killed, and samples of adipose tissue were dissected from a subset of OT-treated (n = 12) and vehicle-treated (n = 12) animals and incubated in culture media for 6 hours. Media samples were analyzed for interleukin (IL)-6 concentration corrected by sample dry weight. Aortas were dissected, formalin-fixed, and stained with oil-red O for en face quantification of lesion area. t tests were used to compare group means on measures of percent lesion area and IL-6 concentrations. RESULTS: There were no group differences in plasma lipids. Adipose tissue samples taken from OT-treated animals secreted significantly less IL-6 over 6 hours (p <.01). OT-treated animals displayed significantly less atherosclerosis in the thoracic aorta (p <.05). CONCLUSIONS: These results indicate that peripheral OT administration can inhibit atherosclerotic lesion development and adipose tissue inflammation, suggesting a potential role for this neuropeptide in mediating the benefits of stable group housing on atherosclerosis.

Original languageEnglish
Pages (from-to)376-382
Number of pages7
JournalPsychosomatic Medicine
Volume72
Issue number4
DOIs
StatePublished - May 1 2010

Fingerprint

Apolipoproteins E
Oxytocin
Adipose Tissue
Atherosclerosis
Inflammation
Interleukin-6
Social Environment
Posterior Pituitary Hormones
Social Behavior
Interpersonal Relations
Neuropeptides
Thoracic Aorta
Formaldehyde
Culture Media
Aorta
Homeostasis
Lipids
Weights and Measures

Keywords

  • Atherosclerosis
  • Inflammation
  • Interleukin-6
  • Oxytocin
  • Visceral adipose tissue

ASJC Scopus subject areas

  • Psychiatry and Mental health
  • Applied Psychology

Cite this

Oxytocin attenuates atherosclerosis and adipose tissue inflammation in socially isolated ApoE-/- Mice. / Nation, Daniel A.; Szeto, Angela; Mendez, Armando J; Brooks, Larry G.; Zaias, Julia; Herderick, Edward E.; Gonzales, Julie; Noller, Crystal M.; Schneiderman, Neil; McCabe, Philip.

In: Psychosomatic Medicine, Vol. 72, No. 4, 01.05.2010, p. 376-382.

Research output: Contribution to journalArticle

Nation, Daniel A. ; Szeto, Angela ; Mendez, Armando J ; Brooks, Larry G. ; Zaias, Julia ; Herderick, Edward E. ; Gonzales, Julie ; Noller, Crystal M. ; Schneiderman, Neil ; McCabe, Philip. / Oxytocin attenuates atherosclerosis and adipose tissue inflammation in socially isolated ApoE-/- Mice. In: Psychosomatic Medicine. 2010 ; Vol. 72, No. 4. pp. 376-382.
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AU - Nation, Daniel A.

AU - Szeto, Angela

AU - Mendez, Armando J

AU - Brooks, Larry G.

AU - Zaias, Julia

AU - Herderick, Edward E.

AU - Gonzales, Julie

AU - Noller, Crystal M.

AU - Schneiderman, Neil

AU - McCabe, Philip

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N2 - OBJECTIVE: To determine the effect of exogenous oxytocin (OT) administration on inflammation and atherosclerosis in socially isolated apoE mice. Hyperlipidemic animals housed in isolated or stressful social environments display more extensive atherosclerosis than those in an affiliative social environment. The neurohypophyseal peptide OT may be involved in both affiliative social behavior and cardiovascular homeostasis, suggesting a role in mediating the benefits of positive social interactions on atherosclerosis. METHODS: A total of 43, 12-week-old, apoE-/- mice were surgically implanted with osmotic minipumps containing OT (n = 23) or vehicle (n = 20). Blood samples were taken at baseline and after 6 weeks and 12 weeks of treatment. After 12 weeks of treatment, animals were killed, and samples of adipose tissue were dissected from a subset of OT-treated (n = 12) and vehicle-treated (n = 12) animals and incubated in culture media for 6 hours. Media samples were analyzed for interleukin (IL)-6 concentration corrected by sample dry weight. Aortas were dissected, formalin-fixed, and stained with oil-red O for en face quantification of lesion area. t tests were used to compare group means on measures of percent lesion area and IL-6 concentrations. RESULTS: There were no group differences in plasma lipids. Adipose tissue samples taken from OT-treated animals secreted significantly less IL-6 over 6 hours (p <.01). OT-treated animals displayed significantly less atherosclerosis in the thoracic aorta (p <.05). CONCLUSIONS: These results indicate that peripheral OT administration can inhibit atherosclerotic lesion development and adipose tissue inflammation, suggesting a potential role for this neuropeptide in mediating the benefits of stable group housing on atherosclerosis.

AB - OBJECTIVE: To determine the effect of exogenous oxytocin (OT) administration on inflammation and atherosclerosis in socially isolated apoE mice. Hyperlipidemic animals housed in isolated or stressful social environments display more extensive atherosclerosis than those in an affiliative social environment. The neurohypophyseal peptide OT may be involved in both affiliative social behavior and cardiovascular homeostasis, suggesting a role in mediating the benefits of positive social interactions on atherosclerosis. METHODS: A total of 43, 12-week-old, apoE-/- mice were surgically implanted with osmotic minipumps containing OT (n = 23) or vehicle (n = 20). Blood samples were taken at baseline and after 6 weeks and 12 weeks of treatment. After 12 weeks of treatment, animals were killed, and samples of adipose tissue were dissected from a subset of OT-treated (n = 12) and vehicle-treated (n = 12) animals and incubated in culture media for 6 hours. Media samples were analyzed for interleukin (IL)-6 concentration corrected by sample dry weight. Aortas were dissected, formalin-fixed, and stained with oil-red O for en face quantification of lesion area. t tests were used to compare group means on measures of percent lesion area and IL-6 concentrations. RESULTS: There were no group differences in plasma lipids. Adipose tissue samples taken from OT-treated animals secreted significantly less IL-6 over 6 hours (p <.01). OT-treated animals displayed significantly less atherosclerosis in the thoracic aorta (p <.05). CONCLUSIONS: These results indicate that peripheral OT administration can inhibit atherosclerotic lesion development and adipose tissue inflammation, suggesting a potential role for this neuropeptide in mediating the benefits of stable group housing on atherosclerosis.

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KW - Oxytocin

KW - Visceral adipose tissue

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