Oxygen exchange and vascular resistance in the totally perfused rainbow trout

C. M. Wood, B. R. McMahon, D. G. McDonald

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

A whole trout preparation (Salmo gairdneri) externally ventilated with water and internally perfused with artificial medium via a cardiac pump is described for the study of O2 exchange and vascular resistance. As cardiac output (Q) was raised, ventral and dorsal aortic pressures increased while branchial (Rg) and systemic (Rs) vascular resistances fell, reflecting considerable passive distensibility. Arterial oxygenation was negative at low Q's due to significant internal O2 demand by the gill tissue, but increased to zero or positive values at intermediate Q's, and eventually declined at high Q's because of transit time limitation. O2 uptake from the ventilatory flow rose with increasing Q. Epinephrine (10-5 M) decreased Rg, increased Rs, and enhanced arterial oxygenation. Artificial elevation of dorsal aortic pressure decreased Rg but did not affect arterial oxygenation. A 10-fold elevation of ventilatory flow increased arterial oxygenation but did not alter Rg or Rs. Endogenous metabolism of branchial tissue accounted for 11.7% of resting O.2 uptake in vivo, and comprised an internal component taking O2 from perfusion flow and an external component drawing O2 from ventilatory flow.

Original languageEnglish
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume3
Issue number3
StatePublished - Dec 1 1978

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Oncorhynchus mykiss
Vascular Resistance
Arterial Pressure
Oxygen
Trout
Cardiac Output
Epinephrine
Perfusion
Water

ASJC Scopus subject areas

  • Physiology

Cite this

Oxygen exchange and vascular resistance in the totally perfused rainbow trout. / Wood, C. M.; McMahon, B. R.; McDonald, D. G.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 3, No. 3, 01.12.1978.

Research output: Contribution to journalArticle

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