Oxygen-derived free radicals and local control of striated muscle blood flow

Kenneth G Proctor, Brian R. Duling

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The role of oxygen-derived free radicals in the local control of blood flow (BF) was evaluated in the microcirculation of the hamster cremaster muscle superfused with the enzymatic radical scavengers superoxide dismutase (SOD, 10 μg/ml) and catalase (CAT, 20 μg/ml). A bioassay of the cheek pouch microcirculation, using a previously described fluorescent microscopic technique, verified the in vivo activity of SOD and CAT. Increased superfusate O2 content to 95% O2 was correlated with an arteriolar vasoconstriction of approximately 50% and a 70% reduction in estimated BF, neither of which was affected by SOD-CAT treatment. These data suggest that free radicals do not mediate the vasoactive effect of O2. SOD-CAT did not alter vascular reactivity because a twofold vasodilation and a fourfold BF increase after topical adenosine application and a fivefold vasoconstriction after topical norepinephrine were unaffected by enzyme treatment. A twofold vasodilation accompanied striated muscle contraction, but there was no effect of SOD-CAT. Assuming SOD and CAT are not excluded from the striated muscle interstitial space, we conclude that the release of superoxide radicals into the extracellular space does not participate in local microvascular control of cremaster muscle BF.

Original languageEnglish
Pages (from-to)77-86
Number of pages10
JournalMicrovascular Research
Volume24
Issue number1
DOIs
StatePublished - Jan 1 1982
Externally publishedYes

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Striated Muscle
Free Radicals
Muscle
Blood
Oxygen
Microcirculation
Abdominal Muscles
Vasoconstriction
Vasodilation
Bioassay
Cheek
Extracellular Space
Muscle Contraction
Superoxides
Cricetinae
Biological Assay
Adenosine
Catalase
Superoxide Dismutase
Blood Vessels

ASJC Scopus subject areas

  • Biochemistry
  • Cardiology and Cardiovascular Medicine

Cite this

Oxygen-derived free radicals and local control of striated muscle blood flow. / Proctor, Kenneth G; Duling, Brian R.

In: Microvascular Research, Vol. 24, No. 1, 01.01.1982, p. 77-86.

Research output: Contribution to journalArticle

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