Oxidative stress differentially modulates phosphorylation of ERK, p38 and CREB induced by NGF or EGF in PC12 cells

Liang Zhang, Richard S Jope

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

This study assessed if oxidative stress induced by treatment of PC12 cells with H2O2 modulated signaling cascades induced by nerve growth factor (NGF) or epidermal growth factor (EGF) because oxidative stress and impaired growth factor function are associated with aging and aging-associated diseases such as Alzheimer's disease. Phosphorylation of extracellular signal-regulated kinases1 and 2 (ERK1/2) and of p38 kinase was rapidly increased after treatment with NGF, EGF, or H2O2, with NGF causing more prolonged increases than the other agents. Pretreatment with H2O2 did not alter phosphorylation of ERK1/2 induced by either growth factor, but increased the phosphorylation of p38 kinase induced by treatment with NGF or EGF alone. CREB phosphorylation at SER 133 was rapidly increased by treatment with either NGF or EGF. Pretreatment with H2O2 reduced CREB phosphorylation induced by either growth factor. This seemed to be a direct effect because H2O2 also inhibited CREB phosphorylation induced by the adenylyl cyclase stimulator forskolin. These results demonstrate that oxidative stress can differentially modulate growth factor-initiated signaling cascades. Furthermore, because CREB is an evolutionarily preserved protein involved in the formation of long term memory, these results indicate a new target of oxidative stress that may be important in disorders involving impaired memory, such as Alzheimer's disease. Copyright (C) 1999 Elsevier Science Inc.

Original languageEnglish
Pages (from-to)271-278
Number of pages8
JournalNeurobiology of Aging
Volume20
Issue number3
DOIs
StatePublished - Jan 1 1999
Externally publishedYes

Fingerprint

PC12 Cells
Nerve Growth Factor
Epidermal Growth Factor
Oxidative Stress
Phosphorylation
Intercellular Signaling Peptides and Proteins
Alzheimer Disease
Phosphotransferases
Long-Term Memory
Colforsin
Adenylyl Cyclases
Proteins

Keywords

  • Alzheimer's disease
  • CREB
  • EGF
  • ERK
  • NGF
  • Oxidative stress
  • p38
  • PC12 cell

ASJC Scopus subject areas

  • Clinical Neurology
  • Biological Psychiatry
  • Developmental Neuroscience
  • Neurology
  • Psychology(all)

Cite this

Oxidative stress differentially modulates phosphorylation of ERK, p38 and CREB induced by NGF or EGF in PC12 cells. / Zhang, Liang; Jope, Richard S.

In: Neurobiology of Aging, Vol. 20, No. 3, 01.01.1999, p. 271-278.

Research output: Contribution to journalArticle

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