Oxidative stress and heat shock stimulate RGS2 expression in 1321N1 astrocytoma cells

Jaroslaw W. Zmijewski, Ling Song, Richard S. Jope, Lualhati Harkins, Charles S. Cobbs

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

RGS2, a regulators of G-protein signaling family member, regulates G-protein signaling and is itself controlled in part by regulated expression. We tested if cell stress regulates RGS2 expression in human astrocytoma 1321N1 cells. Treatment with H2O2 increased RGS2 mRNA levels time- and concentration-dependently, with 200 μM H2O2 causing an approximately eightfold increase after 2 h. Peroxynitrite and heat shock also increased RGS2 mRNA levels. H2O2-induced RGS2 expression was negatively regulated by phosphoinositide-3-kinase and extracellular signal-regulated kinases. H2O2 also concentration-dependently increased RGS2 protein levels, and the RGS2 appeared to be predominantly in the nucleus. These results demonstrate that RGS2 expression is up-regulated by cell stress.

Original languageEnglish (US)
Pages (from-to)192-196
Number of pages5
JournalArchives of Biochemistry and Biophysics
Volume392
Issue number2
DOIs
StatePublished - Aug 15 2001
Externally publishedYes

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Keywords

  • G-proteins
  • Heat shock
  • Oxidative stress
  • RGS

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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