Oxidant production and immune response after stretch injury in skeletal muscle

S. Brickson, J. Hollander, D. T. Corr, L. L. Ji, Thomas Best

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Purpose: This study investigated oxidant production and associated immune response after acute muscle stretch injury. Methods: A standardized single stretch injury was performed on the tibialis anterior (TA) muscle of 36 male New Zealand white rabbits while contralateral control limbs underwent a sham surgery. Animals were sacrificed 0, 4, 12, 24, 48, and 72 h after injury. Potential sites of oxidant production, measured with a dichlorofluorescein (DCF) probe, were evaluated using two separate buffers. Results: Nonmitochondrial oxidant production measured under basal buffer conditions (0.1 M potassium phosphate) was increased in both injured and control limbs at 24 h (P < 0.01) and was greater in the injured limb at 12 and 48 h (P < 0.01). There was also an interaction of time and injury (P < 0.05). Maximum oxidant production by neutrophils and macrophages, stimulated by the induced buffer (including 1.7 mM ADP, 0.1 mM NADPH, 0.1 mM FeCl3), was increased in both injured and control limbs at 4 h (P < 0.01) and was greater in the injured limb at 48 h (P < 0.01). Myeloperoxidase (MPO) activity, indicating the presence of activated neutrophils, was higher in the injured limb at 4 and 48 h (P < 0.01). The activities of superoxide radical producing and quenching enzymes, xanthine oxidase (XO) and superoxide dismutase (SOD), were elevated at 24 (P < 0.01) and 4 h (P < 0.05), respectively, but showed no difference between injured and control limbs. Conclusion: We conclude that acute muscle stretch injury and the required surgeries to generate the injury result in a biphasic increase in oxidant production in both injured and control limbs, suggesting a systemic immune response. The increase in oxidant production at 4 h may be caused by an increase in activated neutrophils, whereas XO activity may contribute to oxidant generation at 24 h.

Original languageEnglish (US)
Pages (from-to)2010-2015
Number of pages6
JournalMedicine and Science in Sports and Exercise
Volume33
Issue number12
DOIs
StatePublished - Jan 1 2001
Externally publishedYes

Fingerprint

Oxidants
Skeletal Muscle
Extremities
Wounds and Injuries
Buffers
Neutrophils
Xanthine Oxidase
Muscles
NADP
Superoxides
Adenosine Diphosphate
Peroxidase
Superoxide Dismutase
Macrophages
Rabbits
Enzymes

Keywords

  • Acute stretch injury
  • Oxidants

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Oxidant production and immune response after stretch injury in skeletal muscle. / Brickson, S.; Hollander, J.; Corr, D. T.; Ji, L. L.; Best, Thomas.

In: Medicine and Science in Sports and Exercise, Vol. 33, No. 12, 01.01.2001, p. 2010-2015.

Research output: Contribution to journalArticle

Brickson, S. ; Hollander, J. ; Corr, D. T. ; Ji, L. L. ; Best, Thomas. / Oxidant production and immune response after stretch injury in skeletal muscle. In: Medicine and Science in Sports and Exercise. 2001 ; Vol. 33, No. 12. pp. 2010-2015.
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