Overexpression of transforming growth factor-α and epidermal growth factor-receptor in idiopathic pulmonary fibrosis

Robert P. Baughman, Elyse E. Lower, Mary Ann Miller, Pablo A. Bejarano, Sue C. Heffelfinger

Research output: Contribution to journalArticle

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Abstract

Background and Aim: A recent transgenic mouse model overexpressing transforming growth factor alpha (TGF-α) led to a phenotype of pulmonary fibrosis. In order to validate this mouse as a model for idiopathic pulmonary fibrosis in humans, we studied the expression of TGF-α in lung tissue of patients with idiopathic pulmonary fibrosis compared to control lung tissue. Methods: Tissue from both groups was obtained from operative specimens and immediately formalin-fixed and paraffin embedded. Contiguous four micron sections were prepared for conventional histochemical staining and staining with antibodies to either TGF-α or the epidermal growth factor-receptor (EGF-R). Immunostaining was performed using the Ventana ES automated immunohistochemistry system. Four cell types were examined (vascular endothelium, bronchial epithelium, type 2 pneumocytes, and fibroblasts) and stain activity was scored on a six point scale. Results: Eleven patients with IPF were compared to seven control subjects. TGF-α immunoreactivity was significantly higher in the IPF patients than in controls in the vascular endothelium, type 2 pneumocytes, and fibroblasts (P < 0.005). [IPF (4(2-4) Median (Range)) than the controls (0.5(0-2), p < 0.0005).] The differences in EGF-R, one of the receptors for TGF-α, between these two patient populations were not as striking. There was a small but significantly greater expression of EGF-R in the bronchial epithelium and type 2 pneumocytes of the IPF patients. Conclusions: TGF-α is overexpressed in patients with IPF, especially in the vascular endothelial cells.

Original languageEnglish
Pages (from-to)57-61
Number of pages5
JournalSarcoidosis Vasculitis and Diffuse Lung Disease
Volume16
Issue number1
StatePublished - Mar 1 1999

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Idiopathic Pulmonary Fibrosis
Transforming Growth Factors
Transforming Growth Factor alpha
Epidermal Growth Factor Receptor
Alveolar Epithelial Cells
Vascular Endothelium
Epithelium
Fibroblasts
Staining and Labeling
Lung
Pulmonary Fibrosis
Paraffin
Formaldehyde
Transgenic Mice
Coloring Agents
Endothelial Cells
Immunohistochemistry
Phenotype
Antibodies
Population

Keywords

  • Epidermal growth factor-receptor
  • Idiopathic pulmonary fibrosis
  • Transforming growth factor-alpha

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Baughman, R. P., Lower, E. E., Miller, M. A., Bejarano, P. A., & Heffelfinger, S. C. (1999). Overexpression of transforming growth factor-α and epidermal growth factor-receptor in idiopathic pulmonary fibrosis. Sarcoidosis Vasculitis and Diffuse Lung Disease, 16(1), 57-61.

Overexpression of transforming growth factor-α and epidermal growth factor-receptor in idiopathic pulmonary fibrosis. / Baughman, Robert P.; Lower, Elyse E.; Miller, Mary Ann; Bejarano, Pablo A.; Heffelfinger, Sue C.

In: Sarcoidosis Vasculitis and Diffuse Lung Disease, Vol. 16, No. 1, 01.03.1999, p. 57-61.

Research output: Contribution to journalArticle

Baughman, Robert P. ; Lower, Elyse E. ; Miller, Mary Ann ; Bejarano, Pablo A. ; Heffelfinger, Sue C. / Overexpression of transforming growth factor-α and epidermal growth factor-receptor in idiopathic pulmonary fibrosis. In: Sarcoidosis Vasculitis and Diffuse Lung Disease. 1999 ; Vol. 16, No. 1. pp. 57-61.
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