Overexpression of the fibroblast growth factor receptor 1 (FGFR1) in a model of spinal cord injury in rats

Barbara Haenzi, Katharina Ger-Barlag, Halima Akhoundzadeh, Thomas H. Hutson, Sean C. Menezes, Mary B Bunge, Lawrence D F Moon

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Spinal cord injury (SCI) is a severe condition that affects many people and results in high health care costs. Therefore, it is essential to find new targets for treatment. The fibroblast growth factor receptor 1 (FGFR1) signalling pathway has a history of being explored for SCI treatment. Several groups have examined the effect of high availability of different FGFR1 ligands at the injury site and reported corticospinal tract (CST) regeneration as well as improved motor functions. In this study, we investigated overexpression of the FGFR1 in rat corticospinal neurons in vivo after injury (unilateral pyramidotomy) and in cerebellar granule neurons (CGNs) in vitro.We show that overexpression of FGFR1 using AAV1 intracortical injections did not increase sprouting of the treated corticospinal tract and did not improve dexterity or walking in a rat model of SCI. Furthermore, we show that overexpression of FGFR1 in vitro resulted in decreased neurite outgrowth compared to control. Thus, our results suggest that the FGFR1 is not a suitable therapeutic target after SCI.

Original languageEnglish (US)
Article numbere0150541
JournalPLoS One
Volume11
Issue number3
DOIs
StatePublished - Mar 1 2016

Fingerprint

Receptor, Fibroblast Growth Factor, Type 1
Spinal Cord Injuries
spinal cord
Rats
rats
Pyramidal Tracts
Neurons
neurons
health care costs
Wounds and Injuries
neurites
Health Care Costs
Walking
Regeneration
sprouting
Health care
Therapeutics
walking
fibroblast growth factor receptor 1
granules

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Haenzi, B., Ger-Barlag, K., Akhoundzadeh, H., Hutson, T. H., Menezes, S. C., Bunge, M. B., & Moon, L. D. F. (2016). Overexpression of the fibroblast growth factor receptor 1 (FGFR1) in a model of spinal cord injury in rats. PLoS One, 11(3), [e0150541]. https://doi.org/10.1371/journal.pone.0150541

Overexpression of the fibroblast growth factor receptor 1 (FGFR1) in a model of spinal cord injury in rats. / Haenzi, Barbara; Ger-Barlag, Katharina; Akhoundzadeh, Halima; Hutson, Thomas H.; Menezes, Sean C.; Bunge, Mary B; Moon, Lawrence D F.

In: PLoS One, Vol. 11, No. 3, e0150541, 01.03.2016.

Research output: Contribution to journalArticle

Haenzi, B, Ger-Barlag, K, Akhoundzadeh, H, Hutson, TH, Menezes, SC, Bunge, MB & Moon, LDF 2016, 'Overexpression of the fibroblast growth factor receptor 1 (FGFR1) in a model of spinal cord injury in rats', PLoS One, vol. 11, no. 3, e0150541. https://doi.org/10.1371/journal.pone.0150541
Haenzi B, Ger-Barlag K, Akhoundzadeh H, Hutson TH, Menezes SC, Bunge MB et al. Overexpression of the fibroblast growth factor receptor 1 (FGFR1) in a model of spinal cord injury in rats. PLoS One. 2016 Mar 1;11(3). e0150541. https://doi.org/10.1371/journal.pone.0150541
Haenzi, Barbara ; Ger-Barlag, Katharina ; Akhoundzadeh, Halima ; Hutson, Thomas H. ; Menezes, Sean C. ; Bunge, Mary B ; Moon, Lawrence D F. / Overexpression of the fibroblast growth factor receptor 1 (FGFR1) in a model of spinal cord injury in rats. In: PLoS One. 2016 ; Vol. 11, No. 3.
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