Overexpression of rat neuronal calcium sensor-1 in rodent NG108-15 cells enhances synapse formation and transmission

Xiao Liang Chen, Zhen Guo Zhong, Shigeru Yokoyama, Christina Bark, Björn Meister, Per Olof Berggren, John Roder, Haruhiro Higashida, Andreas Jeromin

Research output: Contribution to journalArticle

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Abstract

1. The role of rat neuronal calcium sensor-1 (NCS-1), a Ca2+-binding protein, in synapse formation and transmitter release was examined in mouse neuroblastoma rat glioma hybrid NG108-15 cells in culture. 2. Wild-type NG108-15 cells expressed rodent NCS-1. Endogenous NCS-1 was partially co-localized with the synaptic protein SNAP-25 at the plasma membrane in both cell bodies and processes, but not with the Golgi marker β-COP, an individual coat subunit of the coatomer complex present on Golgi-derived vesicles. 3. In NG108-15 cells co-cultured with rat myotubes, partial co-localization of SNAP-25 and NCS-1 was observed at the plasma membrane of neurites and growth cones, some of which had synaptic contacts to muscle cells. 4. Transient co-transfection of the rat NCS-1 cDNA and green fluorescent protein (GFP) resulted in NCS-1 overexpression in about 30% of the cells as determined by fluorescence microscopy. 5. The rate of functional synapse formation with co-cultured rat myotubes increased 2-fold as determined by the presence of miniature endplate potentials (MEPPs) in NCS-1-overexpressing NG108-15 cells compared to non- and mock-transfected cells. 6. The number of neurites per cell, branches per neurite and length of neurites was slightly less in cells that were either transiently transfected (GFP-NCS-1-fluorescence positive) or stably transformed with NCS-1 compared to GFP-NCS-1-negative, non-transfected or mock-transfected NG108-15 cells. 7. The number of action potentials that elicited endplate potentials increased in NG108-15 cells stably transformed with rat NCS-1. The mean number of quanta per impulse (m) increased 5-fold. 8. These results show that NCS-1 functions to facilitate synapse formation, probably because of the increased quantal content of evoked acetylcholine release.

Original languageEnglish
Pages (from-to)649-659
Number of pages11
JournalJournal of Physiology
Volume532
Issue number3
DOIs
StatePublished - May 1 2001
Externally publishedYes

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Synapses
Rodentia
Neurites
Green Fluorescent Proteins
Skeletal Muscle Fibers
frequenin calcium sensor proteins
Synaptosomal-Associated Protein 25
Cell Membrane
Growth Cones
Neuroblastoma
Fluorescence Microscopy
Glioma
Muscle Cells
Action Potentials
Acetylcholine
Transfection
Cultured Cells
Carrier Proteins
Complementary DNA
Cell Culture Techniques

ASJC Scopus subject areas

  • Physiology

Cite this

Chen, X. L., Zhong, Z. G., Yokoyama, S., Bark, C., Meister, B., Berggren, P. O., ... Jeromin, A. (2001). Overexpression of rat neuronal calcium sensor-1 in rodent NG108-15 cells enhances synapse formation and transmission. Journal of Physiology, 532(3), 649-659. https://doi.org/10.1111/j.1469-7793.2001.0649e.x

Overexpression of rat neuronal calcium sensor-1 in rodent NG108-15 cells enhances synapse formation and transmission. / Chen, Xiao Liang; Zhong, Zhen Guo; Yokoyama, Shigeru; Bark, Christina; Meister, Björn; Berggren, Per Olof; Roder, John; Higashida, Haruhiro; Jeromin, Andreas.

In: Journal of Physiology, Vol. 532, No. 3, 01.05.2001, p. 649-659.

Research output: Contribution to journalArticle

Chen, XL, Zhong, ZG, Yokoyama, S, Bark, C, Meister, B, Berggren, PO, Roder, J, Higashida, H & Jeromin, A 2001, 'Overexpression of rat neuronal calcium sensor-1 in rodent NG108-15 cells enhances synapse formation and transmission', Journal of Physiology, vol. 532, no. 3, pp. 649-659. https://doi.org/10.1111/j.1469-7793.2001.0649e.x
Chen, Xiao Liang ; Zhong, Zhen Guo ; Yokoyama, Shigeru ; Bark, Christina ; Meister, Björn ; Berggren, Per Olof ; Roder, John ; Higashida, Haruhiro ; Jeromin, Andreas. / Overexpression of rat neuronal calcium sensor-1 in rodent NG108-15 cells enhances synapse formation and transmission. In: Journal of Physiology. 2001 ; Vol. 532, No. 3. pp. 649-659.
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AU - Meister, Björn

AU - Berggren, Per Olof

AU - Roder, John

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