Overexpression of laminin-8 in human dermal microvascular endothelial cells promotes angiogenesis-related functions

Jie Li, Lisa Zhou, Hoang T. Tran, Yi Chen, Ngon E. Nguyen, Marvin A. Karasek, M. Peter Marinkovich

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

This study examined the effects of endogenous overexpression of laminin-8 on angiogenesis and wound healing in primary human dermal microvascular endothelial cells (HDMECs). HDMECs expressed laminin-8 and laminin-10, but no other laminins, as determined by radioimmunoprecipitation assay using a panel of antibodies to individual laminin chains. To study laminin-8 function, full-length human laminin α4 cDNA was retrovirally transferred to HDMEC, and specific overexpression of laminin-8 was verified by Western blot. Laminin-8 overexpression promoted endothelial cell spreading and migration in scratch assays and accelerated angiogenic tubule formation in collagen gel overlay assays. Strong inhibitory effect of β1 integrin and weak inhibition by αvβ3 integrin antibodies were observed in laminin-8-stimulated cell migration, but only β1 integrin antibodies affected tubule formation. These studies suggest that laminin-8 overexpression may prove to be a useful method to engineer HDMECs to promote angiogenesis and wound repair.

Original languageEnglish
Pages (from-to)432-440
Number of pages9
JournalJournal of Investigative Dermatology
Volume126
Issue number2
DOIs
StatePublished - Feb 1 2006

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Endothelial cells
Endothelial Cells
Skin
Laminin
Integrins
Assays
Cell Movement
Antibodies
Radioimmunoprecipitation Assay
laminin 8
Wound Healing
Repair
Collagen
Complementary DNA
Western Blotting
Gels
Engineers
Wounds and Injuries

ASJC Scopus subject areas

  • Dermatology

Cite this

Overexpression of laminin-8 in human dermal microvascular endothelial cells promotes angiogenesis-related functions. / Li, Jie; Zhou, Lisa; Tran, Hoang T.; Chen, Yi; Nguyen, Ngon E.; Karasek, Marvin A.; Marinkovich, M. Peter.

In: Journal of Investigative Dermatology, Vol. 126, No. 2, 01.02.2006, p. 432-440.

Research output: Contribution to journalArticle

Li, Jie ; Zhou, Lisa ; Tran, Hoang T. ; Chen, Yi ; Nguyen, Ngon E. ; Karasek, Marvin A. ; Marinkovich, M. Peter. / Overexpression of laminin-8 in human dermal microvascular endothelial cells promotes angiogenesis-related functions. In: Journal of Investigative Dermatology. 2006 ; Vol. 126, No. 2. pp. 432-440.
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