Overexpression of KAI1 suppresses in vitro invasiveness and in vivo metastasis in breast cancer cells

Xiaohong Yang, Lisa L. Wei, Careen Tang, Rebecca Slack, Susette Mueller, Marc E. Lippman

Research output: Contribution to journalArticle

115 Scopus citations

Abstract

KAI1 is a metastasis suppressor gene for human prostate cancer and is also involved in the progression of a variety of other human cancers. Previously, we have demonstrated that KAI1 expression was down-regulated in metastatic breast cancer cell lines as well as in highly aggressive breast cancer specimens. To determine whether KAI1 expression is responsible for the metastasis suppression in breast cancer, we transfected the human KAI1 cDNA into two highly malignant breast cancer cell lines, LCC6 and MDA-MB-231, which both have low levels of endogenous KAI1 expression. Parental, vector-only transfectants and KAI1 transfectant clones were injected into the mammary fat pads and tail veins, respectively, of athymic nude mice and assessed for both spontaneous and experimental lung metastasis. High KAI1 expression significantly suppressed the metastatic potential of KAI1-transfected LCC6 cells. Metastasis suppression correlated with the reduced rate of tumor growth and a decreased clonogenicity in soft agar. Furthermore, KAI1 expression significantly suppressed the in vitro cell invasion in KAI1-transfected MDA-MB-231 cells. Our results suggested that KAI1 may function as a negative regulator of breast cancer metastasis.

Original languageEnglish (US)
Pages (from-to)5284-5288
Number of pages5
JournalCancer Research
Volume61
Issue number13
StatePublished - Jul 1 2001

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Yang, X., Wei, L. L., Tang, C., Slack, R., Mueller, S., & Lippman, M. E. (2001). Overexpression of KAI1 suppresses in vitro invasiveness and in vivo metastasis in breast cancer cells. Cancer Research, 61(13), 5284-5288.