Overexpression and cytoplasmic localization of caspase-6 is associated with lamin A degradation in set of ovarian cancers

Callinice D. Capo-Chichi, Kathy Q. Cai, Xiangxi Xu

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Background: In most women with ovarian cancer, the diagnosis occurs after dissemination of tumor cells beyond ovaries. Several molecular perturbations occur ahead of tumor initiation including loss of lamin A/C. Our hypothesis was that the loss of nuclear structural proteins A type lamins (lamin A/C) transcribed from LMNA gene and substrate for active caspase-6 maybe one of the molecular perturbations. Our objective is to investigate the association between the loss of lamin A/C and the overexpression of caspase-6 in ovarian cancer cells. Method: Western blotting and immunofluorescence were used to analyze the expression of lamin A/C and active caspase-6 in normal human ovarian surface epithelial (HOSE) cells, immortalized human ovarian surface epithelial cells and a set of seven ovarian cancer cell lines (including OVCAR3, OVCAR5, and A2780). The activity of caspase-6 was measured by densitometry, fluorescence and flow cytometry. Immunohistochemistry was used to evaluate the expression of caspase-6 in set of ovarian cancer tissues previously reported to have lost lamin A/C. Results: The results showed that HOSE cells expressed lamin A/C and no or low level of active caspase-6 while cancer cells highly expressed caspase-6 and no or low level of lamin A/C. The inhibition of caspase-6 activity in OVCAR3 cells increased lamin A but has no effect on lamin C; active caspase-6 was localized in the cytoplasm associated with the loss of lamin A. Conclusion: Overexpression and cytoplasmic localization of caspase-6 in ovarian cancer cells may be involved in lamin A degradation and deficiency observed in some ovarian cancer cells.

Original languageEnglish (US)
Article number30
JournalBiomarker Research
Volume6
Issue number1
DOIs
StatePublished - Oct 30 2018

Keywords

  • Active caspase-6
  • Cytoplasmic localization
  • Flow cytometry
  • Immunofluorescence
  • Lamin A/C degradation
  • Ovarian cancer

ASJC Scopus subject areas

  • Molecular Medicine
  • Clinical Biochemistry
  • Biochemistry, medical

Fingerprint Dive into the research topics of 'Overexpression and cytoplasmic localization of caspase-6 is associated with lamin A degradation in set of ovarian cancers'. Together they form a unique fingerprint.

  • Cite this