Ovarian cancer spheroids use myosin-generated force to clear the mesothelium

Marcin P. Iwanicki, Rachel A. Davidowitz, Mei Rosa Ng, Achim Besser, Taru Muranen, Melissa Merritt, Gaudenz Danuser, Tan Ince, Joan S. Brugge

Research output: Contribution to journalArticle

131 Citations (Scopus)

Abstract

Dissemination of ovarian tumors involves the implantation of cancer spheroids into the mesothelial monolayer on the walls of peritoneal and pleural cavity organs. Biopsies of tumors attached to peritoneal organs show that mesothelial cells are not present under tumor masses. We have developed a live, image-based in vitro model in which interactions between tumor spheroids and mesothelial cells can be monitored in real time to provide spatial and temporal understanding of mesothelial clearance. In this article, we provide evidence that ovarian cancer spheroids use integrin- and talin-dependent activation of myosin and traction force to promote displacement of mesothelial cells from underneath a tumor cell spheroid. These results suggest that ovarian tumor cell clusters gain access to the submesothelial environment by exerting force on the mesothelial cells lining target organs, driving migration and clearance of the mesothelial cells. Significance: This study uses time-lapse video microscopy to decipher cellular events associated with ovarian tumor cell intercalation of mesothelial cell layers. Ovarian cancer clusters were found to use actomyosin-generated force to physically displace mesothelial cells and gain access to the submesothelial environment. Blockade of force-conducting molecules, including α 5 integrin, talin I, and nonmuscle myosin II, in cancer cells abrogated mesothelial displacement from underneath attached cancer spheroids.

Original languageEnglish
Pages (from-to)144-157
Number of pages14
JournalCancer Discovery
Volume1
Issue number2
DOIs
StatePublished - Jun 14 2011

Fingerprint

Myosins
Ovarian Neoplasms
Epithelium
Neoplasms
Talin
Integrins
Myosin Type II
Pleural Cavity
Video Microscopy
Actomyosin
Peritoneal Cavity
Traction
Biopsy

ASJC Scopus subject areas

  • Oncology

Cite this

Iwanicki, M. P., Davidowitz, R. A., Ng, M. R., Besser, A., Muranen, T., Merritt, M., ... Brugge, J. S. (2011). Ovarian cancer spheroids use myosin-generated force to clear the mesothelium. Cancer Discovery, 1(2), 144-157. https://doi.org/10.1158/2159-8274.CD-11-0010

Ovarian cancer spheroids use myosin-generated force to clear the mesothelium. / Iwanicki, Marcin P.; Davidowitz, Rachel A.; Ng, Mei Rosa; Besser, Achim; Muranen, Taru; Merritt, Melissa; Danuser, Gaudenz; Ince, Tan; Brugge, Joan S.

In: Cancer Discovery, Vol. 1, No. 2, 14.06.2011, p. 144-157.

Research output: Contribution to journalArticle

Iwanicki, MP, Davidowitz, RA, Ng, MR, Besser, A, Muranen, T, Merritt, M, Danuser, G, Ince, T & Brugge, JS 2011, 'Ovarian cancer spheroids use myosin-generated force to clear the mesothelium', Cancer Discovery, vol. 1, no. 2, pp. 144-157. https://doi.org/10.1158/2159-8274.CD-11-0010
Iwanicki MP, Davidowitz RA, Ng MR, Besser A, Muranen T, Merritt M et al. Ovarian cancer spheroids use myosin-generated force to clear the mesothelium. Cancer Discovery. 2011 Jun 14;1(2):144-157. https://doi.org/10.1158/2159-8274.CD-11-0010
Iwanicki, Marcin P. ; Davidowitz, Rachel A. ; Ng, Mei Rosa ; Besser, Achim ; Muranen, Taru ; Merritt, Melissa ; Danuser, Gaudenz ; Ince, Tan ; Brugge, Joan S. / Ovarian cancer spheroids use myosin-generated force to clear the mesothelium. In: Cancer Discovery. 2011 ; Vol. 1, No. 2. pp. 144-157.
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