Outer membrane protein A, peptidoglycan-associated lipoprotein, and murein lipoprotein are released by Escherichia coli bacteria into serum

Judith Hellman, Paul M. Loiselle, Megan M. Tehan, Jennifer E. Allaire, Lenora A. Boyle, James T. Kurnick, David Andrews, Kwang Sik Kim, H. Shaw Warren

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Complexes containing lipopolysaccharide (LPS) and three outer membrane proteins (OMPs) are released by gram-negative bacteria incubated in human serum and into the circulation in an experimental model of sepsis. The same OMPs are bound by immunoglobulin G (IgG) in the cross-protective antiserum raised to Escherichia coli J5 (anti-J5 IgG). This study was performed to identify the three OMPs. The 35-kDa OMP was identified as outer membrane protein A (OmpA) by immunoblotting studies using OmpA-deficient bacteria and recombinant OmpA protein. The 18-kDa OMP was identified as peptidoglycan- associated lipoprotein (PAL) based on peptide sequences from the purified protein and immunoblotting studies using PAL-deficient bacteria. The 5- to 9- kDa OMP was identified as murein lipoprotein (MLP) based on immunoblotting studies using MLP-deficient bacteria. The studies identify the OMPs released into human serum and into the circulation in an experimental model of sepsis as OmpA, PAL, and MLP.

Original languageEnglish
Pages (from-to)2566-2572
Number of pages7
JournalInfection and Immunity
Volume68
Issue number5
DOIs
StatePublished - May 1 2000
Externally publishedYes

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Peptidoglycan
Lipoproteins
Membrane Proteins
Escherichia coli
Bacteria
Serum
Immunoblotting
Sepsis
Theoretical Models
Immunoglobulin G
B-Cell Antigen Receptors
OMPA outer membrane proteins
Gram-Negative Bacteria
Lipopolysaccharides
Immune Sera
Proteins
Peptides

ASJC Scopus subject areas

  • Immunology

Cite this

Outer membrane protein A, peptidoglycan-associated lipoprotein, and murein lipoprotein are released by Escherichia coli bacteria into serum. / Hellman, Judith; Loiselle, Paul M.; Tehan, Megan M.; Allaire, Jennifer E.; Boyle, Lenora A.; Kurnick, James T.; Andrews, David; Kim, Kwang Sik; Warren, H. Shaw.

In: Infection and Immunity, Vol. 68, No. 5, 01.05.2000, p. 2566-2572.

Research output: Contribution to journalArticle

Hellman, Judith ; Loiselle, Paul M. ; Tehan, Megan M. ; Allaire, Jennifer E. ; Boyle, Lenora A. ; Kurnick, James T. ; Andrews, David ; Kim, Kwang Sik ; Warren, H. Shaw. / Outer membrane protein A, peptidoglycan-associated lipoprotein, and murein lipoprotein are released by Escherichia coli bacteria into serum. In: Infection and Immunity. 2000 ; Vol. 68, No. 5. pp. 2566-2572.
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