Otopathogenic Staphylococcus aureus Invades Human Middle Ear Epithelial Cells Primarily through Cholesterol Dependent Pathway

Rahul Mittal, Luca H. Debs, Amit P. Patel, Desiree Nguyen, Patricia Blackwelder, Denise Yan, Paulo H. Weckwerth, Xue Z Liu

Research output: Contribution to journalArticle

Abstract

Chronic suppurative otitis media (CSOM) is one of the most common infectious diseases of the middle ear especially affecting children, leading to delay in language development and communication. Although Staphylococcus aureus is the most common pathogen associated with CSOM, its interaction with middle ear epithelial cells is not well known. In the present study, we observed that otopathogenic S. aureus has the ability to invade human middle ear epithelial cells (HMEECs) in a dose and time dependent manner. Scanning electron microscopy demonstrated time dependent increase in the number of S. aureus on the surface of HMEECs. We observed that otopathogenic S. aureus primarily employs a cholesterol dependent pathway to colonize HMEECs. In agreement with these findings, confocal microscopy showed that S. aureus colocalized with lipid rafts in HMEECs. The results of the present study provide new insights into the pathogenesis of S. aureus induced CSOM. The availability of in vitro cell culture model will pave the way to develop novel effective treatment modalities for CSOM beyond antibiotic therapy.

Original languageEnglish (US)
Article number10777
JournalScientific reports
Volume9
Issue number1
DOIs
StatePublished - Dec 1 2019

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Middle Ear
Suppurative Otitis Media
Staphylococcus aureus
Epithelial Cells
Cholesterol
Language Development
Aptitude
Confocal Microscopy
Electron Scanning Microscopy
Communicable Diseases
Cell Culture Techniques
Communication
Anti-Bacterial Agents
Lipids
Therapeutics

ASJC Scopus subject areas

  • General

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Otopathogenic Staphylococcus aureus Invades Human Middle Ear Epithelial Cells Primarily through Cholesterol Dependent Pathway. / Mittal, Rahul; Debs, Luca H.; Patel, Amit P.; Nguyen, Desiree; Blackwelder, Patricia; Yan, Denise; Weckwerth, Paulo H.; Liu, Xue Z.

In: Scientific reports, Vol. 9, No. 1, 10777, 01.12.2019.

Research output: Contribution to journalArticle

Mittal, Rahul ; Debs, Luca H. ; Patel, Amit P. ; Nguyen, Desiree ; Blackwelder, Patricia ; Yan, Denise ; Weckwerth, Paulo H. ; Liu, Xue Z. / Otopathogenic Staphylococcus aureus Invades Human Middle Ear Epithelial Cells Primarily through Cholesterol Dependent Pathway. In: Scientific reports. 2019 ; Vol. 9, No. 1.
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