Osteogenic differentiation of stem cells derived from human periodontal ligaments and pulp of human exfoliated deciduous teeth

Kiranmai Chadipiralla, Ji Min Yochim, Bindu Bahuleyan, Chun-Yuh Huang, Franklin Garcia-Godoy, Peter E. Murray, Eric J. Stelnicki

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Multipotent stem cells derived from periodontal ligaments (PDLSC) and pulp of human exfoliated deciduous teeth (SHED) represent promising cell sources for bone regeneration. Recent studies have demonstrated that retinoic acid (RA) and dexamethasone (Dex) induce osteogenesis of postnatal stem cells. The objective of this study was to examine the effects of RA and Dex on the proliferation and osteogenic differentiation of SHED and PDLSC and to compare the osteogenic characteristics of SHED and PDLSC under RA treatment. SHED and PDLSC were treated with serum-free medium either alone or supplemented with RA or Dex for 21 days. The proliferation of SHED and PDLSC was significantly inhibited by both RA and Dex. RA significantly upregulated gene expression and the activity of alkaline phosphatase in SHED and PDLSC. Positive Alizarin red and von Kossa staining of calcium deposition was seen on the RA-treated SHED and PDLSC after 21 days of culture. The influences of RA on the osteogenic differentiation of SHED and PDLSC were significantly stronger than with Dex. Supplemention with insulin enhanced RA-induced osteogenic differentiation of SHED. Thus, RA is an effective inducer of osteogenic differentiation of SHED and PDLSC, whereas RA treatment in combination with insulin supplementation might be a better option for inducing osteogenic differentiation. Significantly higher cell proliferation of PDLSC results in greater calcium deposition after 3-week culture, suggesting that PDLSC is a better osteogenic stem cell source. This study provides valuable information for efficiently producing osteogenically differentiated SHED or PDLSC for in vivo bone regeneration.

Original languageEnglish
Pages (from-to)323-333
Number of pages11
JournalCell and Tissue Research
Volume340
Issue number2
DOIs
StatePublished - May 1 2010

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Periodontal Ligament
Deciduous Tooth
Tretinoin
Stem Cells
Dexamethasone
Bone Regeneration
Insulin
Multipotent Stem Cells
Calcium
Serum-Free Culture Media
Osteogenesis
Alkaline Phosphatase
Cell Proliferation
Staining and Labeling

Keywords

  • Adult stem cells
  • Dental pulp
  • Human
  • Osteogenesis
  • Periodontal ligament
  • Retinoic acid

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Cell Biology
  • Histology

Cite this

Osteogenic differentiation of stem cells derived from human periodontal ligaments and pulp of human exfoliated deciduous teeth. / Chadipiralla, Kiranmai; Yochim, Ji Min; Bahuleyan, Bindu; Huang, Chun-Yuh; Garcia-Godoy, Franklin; Murray, Peter E.; Stelnicki, Eric J.

In: Cell and Tissue Research, Vol. 340, No. 2, 01.05.2010, p. 323-333.

Research output: Contribution to journalArticle

Chadipiralla, Kiranmai ; Yochim, Ji Min ; Bahuleyan, Bindu ; Huang, Chun-Yuh ; Garcia-Godoy, Franklin ; Murray, Peter E. ; Stelnicki, Eric J. / Osteogenic differentiation of stem cells derived from human periodontal ligaments and pulp of human exfoliated deciduous teeth. In: Cell and Tissue Research. 2010 ; Vol. 340, No. 2. pp. 323-333.
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