Osteogenic differentiation of human amniotic fluid-derived stem cells induced by bone morphogenetic protein-7 and enhanced by nanofibrous scaffolds

Hongli Sun, Kai Feng, Jiang Hu, Shay Soker, Anthony Atala, Peter X. Ma

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

Amniotic fluid-derived stem cells (AFSCs) are becoming an important source of cells for regenerative medicine given their apparent advantages of accessibility, renewal capacity and multipotentiality. In the intermediate stage between the embryonic stem cells (ESCs) and adult stem cells, AFSCs may have a distinct mechanism to choose their fate. Unfortunately, until now, little is known about how bone morphogenetic proteins (BMPs) control the osteoblastic differentiation of AFSCs, especially on 3D scaffolds. Our research shows that human AFSCs (hAFSCs) can be induced for osteoblastic differentiation by rhBMP-7, and hAFSCs respond to rhBMP-7 more strongly than human mesenchymal stem cells (hMSCs). As synthetic ECM, scaffolds play a central role in tissue engineering. The hAFSCs, on the nanofibrous scaffolds (NF scaffolds) with morphology similar to that of natural collagen fibers, showed significantly enhanced alkaline phosphatase (ALP) activity, calcium content, von Kossa staining and the expression of osteogenic genes than those on the traditional scaffolds, i.e. solid walled scaffolds. The data on the bone formation in vivo presented further evidence that biomimetic NF scaffolds provided hAFSCs a more favorable synthetic ECM, and thus, facilitated the osteogenic differentiation of hAFSCs. The relative strong responsiveness to rhBMP-7 makes hAFSCs promising in bone regeneration. The synthetic NF scaffolds, which mimic the morphology of natural collagen fibers, enhanced the osteoblastic differentiation of hAFSCs in vitro and bone formation in vivo.

Original languageEnglish
Pages (from-to)1133-1139
Number of pages7
JournalBiomaterials
Volume31
Issue number6
DOIs
StatePublished - Feb 1 2010
Externally publishedYes

Fingerprint

Bone Morphogenetic Protein 7
Amniotic Fluid
Scaffolds (biology)
Stem cells
Scaffolds
Bone
Stem Cells
Proteins
Fluids
Military electronic countermeasures
Collagen
Osteogenesis
Bone Morphogenetic Proteins
Fibers
Phosphatases
Biomimetics
Adult Stem Cells
Bone Regeneration
Regenerative Medicine
Tissue engineering

Keywords

  • Bone
  • Bone morphogenetic protein
  • Extracellular matrix
  • Human amniotic fluid-derived stem cell
  • Nanofiber
  • Tissue engineering scaffold

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Osteogenic differentiation of human amniotic fluid-derived stem cells induced by bone morphogenetic protein-7 and enhanced by nanofibrous scaffolds. / Sun, Hongli; Feng, Kai; Hu, Jiang; Soker, Shay; Atala, Anthony; Ma, Peter X.

In: Biomaterials, Vol. 31, No. 6, 01.02.2010, p. 1133-1139.

Research output: Contribution to journalArticle

Sun, Hongli ; Feng, Kai ; Hu, Jiang ; Soker, Shay ; Atala, Anthony ; Ma, Peter X. / Osteogenic differentiation of human amniotic fluid-derived stem cells induced by bone morphogenetic protein-7 and enhanced by nanofibrous scaffolds. In: Biomaterials. 2010 ; Vol. 31, No. 6. pp. 1133-1139.
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