Abstract
Amniotic fluid-derived stem cells (AFSCs) are becoming an important source of cells for regenerative medicine given their apparent advantages of accessibility, renewal capacity and multipotentiality. In the intermediate stage between the embryonic stem cells (ESCs) and adult stem cells, AFSCs may have a distinct mechanism to choose their fate. Unfortunately, until now, little is known about how bone morphogenetic proteins (BMPs) control the osteoblastic differentiation of AFSCs, especially on 3D scaffolds. Our research shows that human AFSCs (hAFSCs) can be induced for osteoblastic differentiation by rhBMP-7, and hAFSCs respond to rhBMP-7 more strongly than human mesenchymal stem cells (hMSCs). As synthetic ECM, scaffolds play a central role in tissue engineering. The hAFSCs, on the nanofibrous scaffolds (NF scaffolds) with morphology similar to that of natural collagen fibers, showed significantly enhanced alkaline phosphatase (ALP) activity, calcium content, von Kossa staining and the expression of osteogenic genes than those on the traditional scaffolds, i.e. solid walled scaffolds. The data on the bone formation in vivo presented further evidence that biomimetic NF scaffolds provided hAFSCs a more favorable synthetic ECM, and thus, facilitated the osteogenic differentiation of hAFSCs. The relative strong responsiveness to rhBMP-7 makes hAFSCs promising in bone regeneration. The synthetic NF scaffolds, which mimic the morphology of natural collagen fibers, enhanced the osteoblastic differentiation of hAFSCs in vitro and bone formation in vivo.
| Original language | English |
|---|---|
| Pages (from-to) | 1133-1139 |
| Number of pages | 7 |
| Journal | Biomaterials |
| Volume | 31 |
| Issue number | 6 |
| DOIs | |
| State | Published - Feb 1 2010 |
| Externally published | Yes |
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Keywords
- Bone
- Bone morphogenetic protein
- Extracellular matrix
- Human amniotic fluid-derived stem cell
- Nanofiber
- Tissue engineering scaffold
ASJC Scopus subject areas
- Biomaterials
- Bioengineering
- Ceramics and Composites
- Mechanics of Materials
- Biophysics
Cite this
Osteogenic differentiation of human amniotic fluid-derived stem cells induced by bone morphogenetic protein-7 and enhanced by nanofibrous scaffolds. / Sun, Hongli; Feng, Kai; Hu, Jiang; Soker, Shay; Atala, Anthony; Ma, Peter X.
In: Biomaterials, Vol. 31, No. 6, 01.02.2010, p. 1133-1139.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Osteogenic differentiation of human amniotic fluid-derived stem cells induced by bone morphogenetic protein-7 and enhanced by nanofibrous scaffolds
AU - Sun, Hongli
AU - Feng, Kai
AU - Hu, Jiang
AU - Soker, Shay
AU - Atala, Anthony
AU - Ma, Peter X.
PY - 2010/2/1
Y1 - 2010/2/1
N2 - Amniotic fluid-derived stem cells (AFSCs) are becoming an important source of cells for regenerative medicine given their apparent advantages of accessibility, renewal capacity and multipotentiality. In the intermediate stage between the embryonic stem cells (ESCs) and adult stem cells, AFSCs may have a distinct mechanism to choose their fate. Unfortunately, until now, little is known about how bone morphogenetic proteins (BMPs) control the osteoblastic differentiation of AFSCs, especially on 3D scaffolds. Our research shows that human AFSCs (hAFSCs) can be induced for osteoblastic differentiation by rhBMP-7, and hAFSCs respond to rhBMP-7 more strongly than human mesenchymal stem cells (hMSCs). As synthetic ECM, scaffolds play a central role in tissue engineering. The hAFSCs, on the nanofibrous scaffolds (NF scaffolds) with morphology similar to that of natural collagen fibers, showed significantly enhanced alkaline phosphatase (ALP) activity, calcium content, von Kossa staining and the expression of osteogenic genes than those on the traditional scaffolds, i.e. solid walled scaffolds. The data on the bone formation in vivo presented further evidence that biomimetic NF scaffolds provided hAFSCs a more favorable synthetic ECM, and thus, facilitated the osteogenic differentiation of hAFSCs. The relative strong responsiveness to rhBMP-7 makes hAFSCs promising in bone regeneration. The synthetic NF scaffolds, which mimic the morphology of natural collagen fibers, enhanced the osteoblastic differentiation of hAFSCs in vitro and bone formation in vivo.
AB - Amniotic fluid-derived stem cells (AFSCs) are becoming an important source of cells for regenerative medicine given their apparent advantages of accessibility, renewal capacity and multipotentiality. In the intermediate stage between the embryonic stem cells (ESCs) and adult stem cells, AFSCs may have a distinct mechanism to choose their fate. Unfortunately, until now, little is known about how bone morphogenetic proteins (BMPs) control the osteoblastic differentiation of AFSCs, especially on 3D scaffolds. Our research shows that human AFSCs (hAFSCs) can be induced for osteoblastic differentiation by rhBMP-7, and hAFSCs respond to rhBMP-7 more strongly than human mesenchymal stem cells (hMSCs). As synthetic ECM, scaffolds play a central role in tissue engineering. The hAFSCs, on the nanofibrous scaffolds (NF scaffolds) with morphology similar to that of natural collagen fibers, showed significantly enhanced alkaline phosphatase (ALP) activity, calcium content, von Kossa staining and the expression of osteogenic genes than those on the traditional scaffolds, i.e. solid walled scaffolds. The data on the bone formation in vivo presented further evidence that biomimetic NF scaffolds provided hAFSCs a more favorable synthetic ECM, and thus, facilitated the osteogenic differentiation of hAFSCs. The relative strong responsiveness to rhBMP-7 makes hAFSCs promising in bone regeneration. The synthetic NF scaffolds, which mimic the morphology of natural collagen fibers, enhanced the osteoblastic differentiation of hAFSCs in vitro and bone formation in vivo.
KW - Bone
KW - Bone morphogenetic protein
KW - Extracellular matrix
KW - Human amniotic fluid-derived stem cell
KW - Nanofiber
KW - Tissue engineering scaffold
UR - http://www.scopus.com/inward/record.url?scp=72149124487&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=72149124487&partnerID=8YFLogxK
U2 - 10.1016/j.biomaterials.2009.10.030
DO - 10.1016/j.biomaterials.2009.10.030
M3 - Article
C2 - 19857889
AN - SCOPUS:72149124487
VL - 31
SP - 1133
EP - 1139
JO - Biomaterials
JF - Biomaterials
SN - 0142-9612
IS - 6
ER -