Abstract
Uptake of myo-inositol by astrocytes in hypertonic medium (440 mosm/kg H2O) was increased near 3-fold after incubation for 24 hours, which continued for 72 hours, as compared with the uptake by cells cultured in isotonic medium (38 nmoles/mg protein). myo-Inositol uptake by astrocytes cultured in hypotonic medium (180 mosm/kg H2O) for periods up to 72 hours was reduced by 74% to 8 to 10 nmoles/mg protein. Astrocytes incubated in either hypotonic or hypertonic medium for 24 hours and then placed in isotonic medium reversed the initial down- or up-regulation of uptake. Activation of chronic RVD and RVI correlates with regulation of myo-inositol uptake. A 30 to 40 mosm/kg H2O deviation from physiological osmolality can influence myo-inositol homeostasis. The intracellular content of myo-inositol in astrocytes in isotonic medium was 25.6 ± 1.3 μg/mg protein (28 mM). This level of myo-inositol is sufficient for this compound to function as an osmoregulator in primary astrocytes and it is likely to contribute to the maintenance of brain volume.
| Original language | English |
|---|---|
| Pages (from-to) | 331-338 |
| Number of pages | 8 |
| Journal | Neurochemical Research |
| Volume | 19 |
| Issue number | 3 |
| DOIs | |
| State | Published - Mar 1 1994 |
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Keywords
- Astrocytes
- cell volume regulation
- myo-inositol content
- myo-inositol uptake
- osmotic regulation
ASJC Scopus subject areas
- Neuroscience(all)
- Biochemistry
Cite this
Osmotic regulation of myo-inositol uptake in primary astrocyte cultures. / Isaacks, Russell E.; Bender, Alexander S.; Kim, Chang Y.; Prieto, Nicole M.; Norenberg, Michael D.
In: Neurochemical Research, Vol. 19, No. 3, 01.03.1994, p. 331-338.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Osmotic regulation of myo-inositol uptake in primary astrocyte cultures
AU - Isaacks, Russell E.
AU - Bender, Alexander S.
AU - Kim, Chang Y.
AU - Prieto, Nicole M.
AU - Norenberg, Michael D
PY - 1994/3/1
Y1 - 1994/3/1
N2 - Uptake of myo-inositol by astrocytes in hypertonic medium (440 mosm/kg H2O) was increased near 3-fold after incubation for 24 hours, which continued for 72 hours, as compared with the uptake by cells cultured in isotonic medium (38 nmoles/mg protein). myo-Inositol uptake by astrocytes cultured in hypotonic medium (180 mosm/kg H2O) for periods up to 72 hours was reduced by 74% to 8 to 10 nmoles/mg protein. Astrocytes incubated in either hypotonic or hypertonic medium for 24 hours and then placed in isotonic medium reversed the initial down- or up-regulation of uptake. Activation of chronic RVD and RVI correlates with regulation of myo-inositol uptake. A 30 to 40 mosm/kg H2O deviation from physiological osmolality can influence myo-inositol homeostasis. The intracellular content of myo-inositol in astrocytes in isotonic medium was 25.6 ± 1.3 μg/mg protein (28 mM). This level of myo-inositol is sufficient for this compound to function as an osmoregulator in primary astrocytes and it is likely to contribute to the maintenance of brain volume.
AB - Uptake of myo-inositol by astrocytes in hypertonic medium (440 mosm/kg H2O) was increased near 3-fold after incubation for 24 hours, which continued for 72 hours, as compared with the uptake by cells cultured in isotonic medium (38 nmoles/mg protein). myo-Inositol uptake by astrocytes cultured in hypotonic medium (180 mosm/kg H2O) for periods up to 72 hours was reduced by 74% to 8 to 10 nmoles/mg protein. Astrocytes incubated in either hypotonic or hypertonic medium for 24 hours and then placed in isotonic medium reversed the initial down- or up-regulation of uptake. Activation of chronic RVD and RVI correlates with regulation of myo-inositol uptake. A 30 to 40 mosm/kg H2O deviation from physiological osmolality can influence myo-inositol homeostasis. The intracellular content of myo-inositol in astrocytes in isotonic medium was 25.6 ± 1.3 μg/mg protein (28 mM). This level of myo-inositol is sufficient for this compound to function as an osmoregulator in primary astrocytes and it is likely to contribute to the maintenance of brain volume.
KW - Astrocytes
KW - cell volume regulation
KW - myo-inositol content
KW - myo-inositol uptake
KW - osmotic regulation
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UR - http://www.scopus.com/inward/citedby.url?scp=0027954640&partnerID=8YFLogxK
U2 - 10.1007/BF00971582
DO - 10.1007/BF00971582
M3 - Article
C2 - 8177373
AN - SCOPUS:0027954640
VL - 19
SP - 331
EP - 338
JO - Neurochemical Research
JF - Neurochemical Research
SN - 0364-3190
IS - 3
ER -