Osmotic regulation of myo-inositol uptake in primary astrocyte cultures

Russell E. Isaacks, Alexander S. Bender, Chang Y. Kim, Nicole M. Prieto, Michael D Norenberg

Research output: Contribution to journalArticle

98 Citations (Scopus)

Abstract

Uptake of myo-inositol by astrocytes in hypertonic medium (440 mosm/kg H2O) was increased near 3-fold after incubation for 24 hours, which continued for 72 hours, as compared with the uptake by cells cultured in isotonic medium (38 nmoles/mg protein). myo-Inositol uptake by astrocytes cultured in hypotonic medium (180 mosm/kg H2O) for periods up to 72 hours was reduced by 74% to 8 to 10 nmoles/mg protein. Astrocytes incubated in either hypotonic or hypertonic medium for 24 hours and then placed in isotonic medium reversed the initial down- or up-regulation of uptake. Activation of chronic RVD and RVI correlates with regulation of myo-inositol uptake. A 30 to 40 mosm/kg H2O deviation from physiological osmolality can influence myo-inositol homeostasis. The intracellular content of myo-inositol in astrocytes in isotonic medium was 25.6 ± 1.3 μg/mg protein (28 mM). This level of myo-inositol is sufficient for this compound to function as an osmoregulator in primary astrocytes and it is likely to contribute to the maintenance of brain volume.

Original languageEnglish
Pages (from-to)331-338
Number of pages8
JournalNeurochemical Research
Volume19
Issue number3
DOIs
StatePublished - Mar 1 1994

Fingerprint

Inositol
Astrocytes
Proteins
Osmolar Concentration
Cultured Cells
Brain
Homeostasis
Up-Regulation
Down-Regulation
Chemical activation
Maintenance

Keywords

  • Astrocytes
  • cell volume regulation
  • myo-inositol content
  • myo-inositol uptake
  • osmotic regulation

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry

Cite this

Osmotic regulation of myo-inositol uptake in primary astrocyte cultures. / Isaacks, Russell E.; Bender, Alexander S.; Kim, Chang Y.; Prieto, Nicole M.; Norenberg, Michael D.

In: Neurochemical Research, Vol. 19, No. 3, 01.03.1994, p. 331-338.

Research output: Contribution to journalArticle

Isaacks, Russell E. ; Bender, Alexander S. ; Kim, Chang Y. ; Prieto, Nicole M. ; Norenberg, Michael D. / Osmotic regulation of myo-inositol uptake in primary astrocyte cultures. In: Neurochemical Research. 1994 ; Vol. 19, No. 3. pp. 331-338.
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