Abstract
Uptake of myo-inositol by astrocytes in hypertonic medium (440 mosm/kg H2O) was increased near 3-fold after incubation for 24 hours, which continued for 72 hours, as compared with the uptake by cells cultured in isotonic medium (38 nmoles/mg protein). myo-Inositol uptake by astrocytes cultured in hypotonic medium (180 mosm/kg H2O) for periods up to 72 hours was reduced by 74% to 8 to 10 nmoles/mg protein. Astrocytes incubated in either hypotonic or hypertonic medium for 24 hours and then placed in isotonic medium reversed the initial down- or up-regulation of uptake. Activation of chronic RVD and RVI correlates with regulation of myo-inositol uptake. A 30 to 40 mosm/kg H2O deviation from physiological osmolality can influence myo-inositol homeostasis. The intracellular content of myo-inositol in astrocytes in isotonic medium was 25.6 ± 1.3 μg/mg protein (28 mM). This level of myo-inositol is sufficient for this compound to function as an osmoregulator in primary astrocytes and it is likely to contribute to the maintenance of brain volume.
Original language | English (US) |
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Pages (from-to) | 331-338 |
Number of pages | 8 |
Journal | Neurochemical Research |
Volume | 19 |
Issue number | 3 |
DOIs | |
State | Published - Mar 1994 |
Keywords
- Astrocytes
- cell volume regulation
- myo-inositol content
- myo-inositol uptake
- osmotic regulation
ASJC Scopus subject areas
- Neuroscience(all)
- Biochemistry