Oscillatory dynamics of Cdc42 GTPase in the control of polarized growth

Maitreyi Das, Tyler Drake, David J. Wiley, Peter Buchwald, Dimitrios Vavylonis, Fulvia Verde

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

Cells promote polarized growth by activation of Rho-family protein Cdc42 at the cell membrane. We combined experiments and modeling to study bipolar growth initiation in fission yeast. Concentrations of a fluorescent marker for active Cdc42, Cdc42 protein, Cdc42-activator Scd1, and scaffold protein Scd2 exhibited anticorrelated fluctuations and oscillations with a 5-minute average period at polarized cell tips. These dynamics indicate competition for active Cdc42 or its regulators and the presence of positive and delayed negative feedbacks. Cdc42 oscillations and spatial distribution were sensitive to the amounts of Cdc42-activator Gef1 and to the activity of Cdc42-dependent kinase Pak1, a negative regulator. Feedbacks regulating Cdc42 oscillations and spatial self-organization appear to provide a flexible mechanism for fission yeast cells to explore polarization states and to control their morphology.

Original languageEnglish
Pages (from-to)239-243
Number of pages5
JournalScience
Volume337
Issue number6091
DOIs
StatePublished - Jul 13 2012

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Cells
Protein
Oscillation
Fission
Activation
Polarization
Experiment
Spatial Distribution
Modeling
Negative Feedback
Fluctuations
Membrane
Self-organization

ASJC Scopus subject areas

  • General
  • History and Philosophy of Science

Cite this

Oscillatory dynamics of Cdc42 GTPase in the control of polarized growth. / Das, Maitreyi; Drake, Tyler; Wiley, David J.; Buchwald, Peter; Vavylonis, Dimitrios; Verde, Fulvia.

In: Science, Vol. 337, No. 6091, 13.07.2012, p. 239-243.

Research output: Contribution to journalArticle

Das, Maitreyi ; Drake, Tyler ; Wiley, David J. ; Buchwald, Peter ; Vavylonis, Dimitrios ; Verde, Fulvia. / Oscillatory dynamics of Cdc42 GTPase in the control of polarized growth. In: Science. 2012 ; Vol. 337, No. 6091. pp. 239-243.
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