Abstract
Cells promote polarized growth by activation of Rho-family protein Cdc42 at the cell membrane. We combined experiments and modeling to study bipolar growth initiation in fission yeast. Concentrations of a fluorescent marker for active Cdc42, Cdc42 protein, Cdc42-activator Scd1, and scaffold protein Scd2 exhibited anticorrelated fluctuations and oscillations with a 5-minute average period at polarized cell tips. These dynamics indicate competition for active Cdc42 or its regulators and the presence of positive and delayed negative feedbacks. Cdc42 oscillations and spatial distribution were sensitive to the amounts of Cdc42-activator Gef1 and to the activity of Cdc42-dependent kinase Pak1, a negative regulator. Feedbacks regulating Cdc42 oscillations and spatial self-organization appear to provide a flexible mechanism for fission yeast cells to explore polarization states and to control their morphology.
| Original language | English |
|---|---|
| Pages (from-to) | 239-243 |
| Number of pages | 5 |
| Journal | Science |
| Volume | 337 |
| Issue number | 6091 |
| DOIs | |
| State | Published - Jul 13 2012 |
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ASJC Scopus subject areas
- General
- History and Philosophy of Science
Cite this
Oscillatory dynamics of Cdc42 GTPase in the control of polarized growth. / Das, Maitreyi; Drake, Tyler; Wiley, David J.; Buchwald, Peter; Vavylonis, Dimitrios; Verde, Fulvia.
In: Science, Vol. 337, No. 6091, 13.07.2012, p. 239-243.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Oscillatory dynamics of Cdc42 GTPase in the control of polarized growth
AU - Das, Maitreyi
AU - Drake, Tyler
AU - Wiley, David J.
AU - Buchwald, Peter
AU - Vavylonis, Dimitrios
AU - Verde, Fulvia
PY - 2012/7/13
Y1 - 2012/7/13
N2 - Cells promote polarized growth by activation of Rho-family protein Cdc42 at the cell membrane. We combined experiments and modeling to study bipolar growth initiation in fission yeast. Concentrations of a fluorescent marker for active Cdc42, Cdc42 protein, Cdc42-activator Scd1, and scaffold protein Scd2 exhibited anticorrelated fluctuations and oscillations with a 5-minute average period at polarized cell tips. These dynamics indicate competition for active Cdc42 or its regulators and the presence of positive and delayed negative feedbacks. Cdc42 oscillations and spatial distribution were sensitive to the amounts of Cdc42-activator Gef1 and to the activity of Cdc42-dependent kinase Pak1, a negative regulator. Feedbacks regulating Cdc42 oscillations and spatial self-organization appear to provide a flexible mechanism for fission yeast cells to explore polarization states and to control their morphology.
AB - Cells promote polarized growth by activation of Rho-family protein Cdc42 at the cell membrane. We combined experiments and modeling to study bipolar growth initiation in fission yeast. Concentrations of a fluorescent marker for active Cdc42, Cdc42 protein, Cdc42-activator Scd1, and scaffold protein Scd2 exhibited anticorrelated fluctuations and oscillations with a 5-minute average period at polarized cell tips. These dynamics indicate competition for active Cdc42 or its regulators and the presence of positive and delayed negative feedbacks. Cdc42 oscillations and spatial distribution were sensitive to the amounts of Cdc42-activator Gef1 and to the activity of Cdc42-dependent kinase Pak1, a negative regulator. Feedbacks regulating Cdc42 oscillations and spatial self-organization appear to provide a flexible mechanism for fission yeast cells to explore polarization states and to control their morphology.
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UR - http://www.scopus.com/inward/citedby.url?scp=84863778973&partnerID=8YFLogxK
U2 - 10.1126/science.1218377
DO - 10.1126/science.1218377
M3 - Article
C2 - 22604726
AN - SCOPUS:84863778973
VL - 337
SP - 239
EP - 243
JO - Science
JF - Science
SN - 0036-8075
IS - 6091
ER -