Abstract
Pancreatic β cells exhibit oscillations in electrical activity, cytoplasmic free Ca2+ concentration ([Ca2+]i), and insulin release upon glucose stimulation. The mechanism by which these oscillations are generated is not known. Here we demonstrate fluctuations in the activity of the ATP-dependent K+ channels (KATP channels) in single β cells subject to glucose stimulation or to stimulation with low concentrations of tolbutamide. During stimulation with glucose or low concentrations of tolbutamide, KATP channel activity decreased and action potentials ensued. After 2-3 min, despite continuous stimulation, action potentials subsided and openings of KATP channels could again be observed. Transient suppression of metabolism by azide in glucose-stimulated β cells caused reversible termination of electrical activity, mimicking the spontaneous changes observed with continuous glucose stimulation. Thus, oscillations in KATP channel activity during continuous glucose stimulation result in oscillations in electrical activity and [Ca2+]i.
| Original language | English |
|---|---|
| Pages (from-to) | 5161-5165 |
| Number of pages | 5 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 93 |
| Issue number | 10 |
| State | Published - May 14 1996 |
| Externally published | Yes |
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ASJC Scopus subject areas
- General
- Genetics
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Oscillations in KATP channel activity promote oscillations in cytoplasmic free Ca2+ concentration in the pancreatic β cell. / Larsson, Olof; Kindmark, Henrik; Bränström, Robert; Fredholm, Bertil; Berggren, Per Olof.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, No. 10, 14.05.1996, p. 5161-5165.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Oscillations in KATP channel activity promote oscillations in cytoplasmic free Ca2+ concentration in the pancreatic β cell
AU - Larsson, Olof
AU - Kindmark, Henrik
AU - Bränström, Robert
AU - Fredholm, Bertil
AU - Berggren, Per Olof
PY - 1996/5/14
Y1 - 1996/5/14
N2 - Pancreatic β cells exhibit oscillations in electrical activity, cytoplasmic free Ca2+ concentration ([Ca2+]i), and insulin release upon glucose stimulation. The mechanism by which these oscillations are generated is not known. Here we demonstrate fluctuations in the activity of the ATP-dependent K+ channels (KATP channels) in single β cells subject to glucose stimulation or to stimulation with low concentrations of tolbutamide. During stimulation with glucose or low concentrations of tolbutamide, KATP channel activity decreased and action potentials ensued. After 2-3 min, despite continuous stimulation, action potentials subsided and openings of KATP channels could again be observed. Transient suppression of metabolism by azide in glucose-stimulated β cells caused reversible termination of electrical activity, mimicking the spontaneous changes observed with continuous glucose stimulation. Thus, oscillations in KATP channel activity during continuous glucose stimulation result in oscillations in electrical activity and [Ca2+]i.
AB - Pancreatic β cells exhibit oscillations in electrical activity, cytoplasmic free Ca2+ concentration ([Ca2+]i), and insulin release upon glucose stimulation. The mechanism by which these oscillations are generated is not known. Here we demonstrate fluctuations in the activity of the ATP-dependent K+ channels (KATP channels) in single β cells subject to glucose stimulation or to stimulation with low concentrations of tolbutamide. During stimulation with glucose or low concentrations of tolbutamide, KATP channel activity decreased and action potentials ensued. After 2-3 min, despite continuous stimulation, action potentials subsided and openings of KATP channels could again be observed. Transient suppression of metabolism by azide in glucose-stimulated β cells caused reversible termination of electrical activity, mimicking the spontaneous changes observed with continuous glucose stimulation. Thus, oscillations in KATP channel activity during continuous glucose stimulation result in oscillations in electrical activity and [Ca2+]i.
UR - http://www.scopus.com/inward/record.url?scp=0029664303&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029664303&partnerID=8YFLogxK
M3 - Article
VL - 93
SP - 5161
EP - 5165
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 10
ER -