Original article

Electrophoretic properties of platelets from normal, thrombotic and ITP patients by doppler electrophoretic light scattering analysis

Wenche Jy, L. L. Horstman, D. Homolak, Yeon Ahn

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

At fixed pH and ionic environment platelet net charge depends on the composition of surface glycoproteins and phospholipids. It is believed that this composition changes, becoming more negative in the course of activation, and that certain thrombotic disorders such as transient ischemic attacks (TIA) may be associated with platelet activation. To investigate this hypothesis we measured the electrophoretic mobility (U) of normal platelets, activated normal platelets and platelets from patients with TIA and immune thrombocytopenic purpura (ITP). Normal platelets gave U == -0.91 ± 0.05 pm cmh, while normal platelets activated by ADP gave -1.25 and by collagen -1.70. TIA patients (n == 22) had mean U == -1.14 ± 0.10 and the ITP patients (n == 37) had U== -1.07 ± 0.13. The U of both patient groups differed significantly from normal controls,P< 0.001. Indeed, only 2 of the 22 TIA cases had U within 2 SD of the control value. The ITP group included 10 patients with TIA-Like symptoms; the mean U of this subgroup was identical to that of the TIA group without ITP. We conclude that U is a useful measure for research on platelet abnormalities and may even be useful as a routine clinical tool. This work was greatly facilitated by the use of an automated instrument, the DELSA 440, allowing determination of U of a given sample in as little as 5 min.

Original languageEnglish
Pages (from-to)354-358
Number of pages5
JournalPlatelets
Volume6
Issue number6
DOIs
StatePublished - Jan 1 1995

Fingerprint

Thrombotic Thrombocytopenic Purpura
Idiopathic Thrombocytopenic Purpura
Transient Ischemic Attack
Blood Platelets
Light
Membrane Glycoproteins
Platelet Activation
Adenosine Diphosphate
Phospholipids
Collagen
Research

ASJC Scopus subject areas

  • Hematology
  • Cell Biology

Cite this

Original article : Electrophoretic properties of platelets from normal, thrombotic and ITP patients by doppler electrophoretic light scattering analysis. / Jy, Wenche; Horstman, L. L.; Homolak, D.; Ahn, Yeon.

In: Platelets, Vol. 6, No. 6, 01.01.1995, p. 354-358.

Research output: Contribution to journalArticle

@article{77e0a27a4d6a4ecf92378d5773acd7c0,
title = "Original article: Electrophoretic properties of platelets from normal, thrombotic and ITP patients by doppler electrophoretic light scattering analysis",
abstract = "At fixed pH and ionic environment platelet net charge depends on the composition of surface glycoproteins and phospholipids. It is believed that this composition changes, becoming more negative in the course of activation, and that certain thrombotic disorders such as transient ischemic attacks (TIA) may be associated with platelet activation. To investigate this hypothesis we measured the electrophoretic mobility (U) of normal platelets, activated normal platelets and platelets from patients with TIA and immune thrombocytopenic purpura (ITP). Normal platelets gave U == -0.91 ± 0.05 pm cmh, while normal platelets activated by ADP gave -1.25 and by collagen -1.70. TIA patients (n == 22) had mean U == -1.14 ± 0.10 and the ITP patients (n == 37) had U== -1.07 ± 0.13. The U of both patient groups differed significantly from normal controls,P< 0.001. Indeed, only 2 of the 22 TIA cases had U within 2 SD of the control value. The ITP group included 10 patients with TIA-Like symptoms; the mean U of this subgroup was identical to that of the TIA group without ITP. We conclude that U is a useful measure for research on platelet abnormalities and may even be useful as a routine clinical tool. This work was greatly facilitated by the use of an automated instrument, the DELSA 440, allowing determination of U of a given sample in as little as 5 min.",
author = "Wenche Jy and Horstman, {L. L.} and D. Homolak and Yeon Ahn",
year = "1995",
month = "1",
day = "1",
doi = "10.3109/09537109509078471",
language = "English",
volume = "6",
pages = "354--358",
journal = "Platelets",
issn = "0953-7104",
publisher = "Informa Healthcare",
number = "6",

}

TY - JOUR

T1 - Original article

T2 - Electrophoretic properties of platelets from normal, thrombotic and ITP patients by doppler electrophoretic light scattering analysis

AU - Jy, Wenche

AU - Horstman, L. L.

AU - Homolak, D.

AU - Ahn, Yeon

PY - 1995/1/1

Y1 - 1995/1/1

N2 - At fixed pH and ionic environment platelet net charge depends on the composition of surface glycoproteins and phospholipids. It is believed that this composition changes, becoming more negative in the course of activation, and that certain thrombotic disorders such as transient ischemic attacks (TIA) may be associated with platelet activation. To investigate this hypothesis we measured the electrophoretic mobility (U) of normal platelets, activated normal platelets and platelets from patients with TIA and immune thrombocytopenic purpura (ITP). Normal platelets gave U == -0.91 ± 0.05 pm cmh, while normal platelets activated by ADP gave -1.25 and by collagen -1.70. TIA patients (n == 22) had mean U == -1.14 ± 0.10 and the ITP patients (n == 37) had U== -1.07 ± 0.13. The U of both patient groups differed significantly from normal controls,P< 0.001. Indeed, only 2 of the 22 TIA cases had U within 2 SD of the control value. The ITP group included 10 patients with TIA-Like symptoms; the mean U of this subgroup was identical to that of the TIA group without ITP. We conclude that U is a useful measure for research on platelet abnormalities and may even be useful as a routine clinical tool. This work was greatly facilitated by the use of an automated instrument, the DELSA 440, allowing determination of U of a given sample in as little as 5 min.

AB - At fixed pH and ionic environment platelet net charge depends on the composition of surface glycoproteins and phospholipids. It is believed that this composition changes, becoming more negative in the course of activation, and that certain thrombotic disorders such as transient ischemic attacks (TIA) may be associated with platelet activation. To investigate this hypothesis we measured the electrophoretic mobility (U) of normal platelets, activated normal platelets and platelets from patients with TIA and immune thrombocytopenic purpura (ITP). Normal platelets gave U == -0.91 ± 0.05 pm cmh, while normal platelets activated by ADP gave -1.25 and by collagen -1.70. TIA patients (n == 22) had mean U == -1.14 ± 0.10 and the ITP patients (n == 37) had U== -1.07 ± 0.13. The U of both patient groups differed significantly from normal controls,P< 0.001. Indeed, only 2 of the 22 TIA cases had U within 2 SD of the control value. The ITP group included 10 patients with TIA-Like symptoms; the mean U of this subgroup was identical to that of the TIA group without ITP. We conclude that U is a useful measure for research on platelet abnormalities and may even be useful as a routine clinical tool. This work was greatly facilitated by the use of an automated instrument, the DELSA 440, allowing determination of U of a given sample in as little as 5 min.

UR - http://www.scopus.com/inward/record.url?scp=0028824304&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028824304&partnerID=8YFLogxK

U2 - 10.3109/09537109509078471

DO - 10.3109/09537109509078471

M3 - Article

VL - 6

SP - 354

EP - 358

JO - Platelets

JF - Platelets

SN - 0953-7104

IS - 6

ER -