Origin of neointimal cells in arteriovenous fistulae: Bone marrow, artery, or the vein itself?

Nikolaos Skartsis, Eddie Manning, Yuntao Wei, Omaida C Velazquez, Zhao-Jun Liu, Pascal Goldschmidt-Clermont, Loay Salman, Arif Asif, Roberto I Vazquez-Padron

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

To elucidate the source of neointimal cells, experimental fistulas were created in Lewis wild-type (WT) and transgenic rats that constitutively expressed the green fluorescent protein (GFP) in all tissues. Arteriovenous fistula (AVFs) were created by anastomosing the left renal vein to the abdominal aorta. The contribution of bone marrow (BM)-derived cells to the AVF neointima was examined in lethally irradiated WT rats that had been rescued with GFP BM cells. Neointimal cells in these chimeric rats were mostly GFP negative indicating the non-BM origin of those cells. Then, the contribution of arterial cells to the AVF neointima was assessed in a fistula made with a GFP aorta that had been implanted orthotopically into a WT rat. Most of the neointimal cells were also GFP negative demonstrating that AVF neointimal cells are not derived from the feeding artery. Finally to study local resident cells contribution to the formation of neointimal lesions, a composite fistula was created by interposing a GFP vein between the renal vein and the aorta in a WT recipient rat. GFP neointimal cells were only found in the transplanted vein. This study suggests that neointimal cells originate from the local resident cells in the venous limb of the fistula.

Original languageEnglish
Pages (from-to)242-248
Number of pages7
JournalSeminars in Dialysis
Volume24
Issue number2
DOIs
StatePublished - Mar 1 2011

Fingerprint

Arteriovenous Fistula
Veins
Arteries
Bone Marrow
Green Fluorescent Proteins
Fistula
Neointima
Renal Veins
Bone Marrow Cells
Aorta
Transgenic Rats
Abdominal Aorta
Extremities

ASJC Scopus subject areas

  • Nephrology

Cite this

Origin of neointimal cells in arteriovenous fistulae : Bone marrow, artery, or the vein itself? / Skartsis, Nikolaos; Manning, Eddie; Wei, Yuntao; Velazquez, Omaida C; Liu, Zhao-Jun; Goldschmidt-Clermont, Pascal; Salman, Loay; Asif, Arif; Vazquez-Padron, Roberto I.

In: Seminars in Dialysis, Vol. 24, No. 2, 01.03.2011, p. 242-248.

Research output: Contribution to journalArticle

Skartsis, Nikolaos ; Manning, Eddie ; Wei, Yuntao ; Velazquez, Omaida C ; Liu, Zhao-Jun ; Goldschmidt-Clermont, Pascal ; Salman, Loay ; Asif, Arif ; Vazquez-Padron, Roberto I. / Origin of neointimal cells in arteriovenous fistulae : Bone marrow, artery, or the vein itself?. In: Seminars in Dialysis. 2011 ; Vol. 24, No. 2. pp. 242-248.
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