Oriented assembly of proteins on surfaces

Leonidas G. Bachas, D. Bhattacharyya, Kimberly W. Anderson

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Several approaches that facilitate the oriented assembly of proteins on a variety of surfaces, including membranes, will be described. Biotinylation reactions performed under controlled conditions were used to immobilize enzymes in a layer-by-layer fashion onto surfaces modified with avidin or streptavidin. For other enzymes, a specific attachment site was introduced by gene fusion or site-directed mutagenesis. These strategies were applied to achieve site-specific immobilization of several enzymes including alkaline phosphatase, organophosphorus hydrolase, horseradish peroxidase, and subtilisin. The site-specific immobilization led to orientation of the enzyme molecules on the surface of materials and to a higher activity compared to conventional immobilization methods.

Original languageEnglish (US)
Title of host publicationAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
PublisherIEEE
Number of pages1
ISBN (Print)0780356756
StatePublished - Dec 1 1999
Externally publishedYes
EventProceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS) - Atlanta, GA, USA
Duration: Oct 13 1999Oct 16 1999

Publication series

NameAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Volume2
ISSN (Print)0589-1019

Other

OtherProceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS)
CityAtlanta, GA, USA
Period10/13/9910/16/99

ASJC Scopus subject areas

  • Signal Processing
  • Biomedical Engineering
  • Computer Vision and Pattern Recognition
  • Health Informatics

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  • Cite this

    Bachas, L. G., Bhattacharyya, D., & Anderson, K. W. (1999). Oriented assembly of proteins on surfaces. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings (Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings; Vol. 2). IEEE.