Orientations of transfer RNA in the ribosomal A and P sites

Thomas R. Easterwood, François Major, Arun Malhotra, Stephen C. Harvey

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

In protein synthesis, peptide bond formation requires that the tRNA carrying the amino acid (A site tRNA) contact the tRNA carrying the growing peptide chain (P site tRNA) at their 3′ termini. Two models have been proposed for the orientations of two tRNAs as they would be bound to the mRNA in the ribosome. Viewing the tRNA as an upside down L, anticodon loop pointing down, acceptor stem pointing right, and calling this the front view, the R (Rich) model would have the back of the P site tRNA facing the front of the A site tRNA. In the S (Sundaralingam) model the front of the P site tRNA faces the back of the A site tRNA. Models of two tRNAs bound to mRNA as they would be positioned in the ribosomal A and P sites have been created using MC-SYM, a constraint satisfaction search program designed to build nucleic acid structures. The models incorporate information from fluorescence energy transfer experiments and chemical crosslinks. The models that best answer the constraints are of the S variety, with no R conformations produced consistent with the constraints.

Original languageEnglish
Pages (from-to)3779-3786
Number of pages8
JournalNucleic Acids Research
Volume22
Issue number18
StatePublished - Sep 11 1994
Externally publishedYes

Fingerprint

Transfer RNA
Peptides
Messenger RNA
Model
Constraint Satisfaction
Protein Synthesis
Energy Transfer
Conformation
Amino Acid-Specific Transfer RNA
Fluorescence
Amino Acids
Anticodon
Contact
Nucleic acids
Ribosomes
Nucleic Acids
Energy transfer
Conformations
Amino acids
Experiment

ASJC Scopus subject areas

  • Genetics
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)

Cite this

Easterwood, T. R., Major, F., Malhotra, A., & Harvey, S. C. (1994). Orientations of transfer RNA in the ribosomal A and P sites. Nucleic Acids Research, 22(18), 3779-3786.

Orientations of transfer RNA in the ribosomal A and P sites. / Easterwood, Thomas R.; Major, François; Malhotra, Arun; Harvey, Stephen C.

In: Nucleic Acids Research, Vol. 22, No. 18, 11.09.1994, p. 3779-3786.

Research output: Contribution to journalArticle

Easterwood, TR, Major, F, Malhotra, A & Harvey, SC 1994, 'Orientations of transfer RNA in the ribosomal A and P sites', Nucleic Acids Research, vol. 22, no. 18, pp. 3779-3786.
Easterwood TR, Major F, Malhotra A, Harvey SC. Orientations of transfer RNA in the ribosomal A and P sites. Nucleic Acids Research. 1994 Sep 11;22(18):3779-3786.
Easterwood, Thomas R. ; Major, François ; Malhotra, Arun ; Harvey, Stephen C. / Orientations of transfer RNA in the ribosomal A and P sites. In: Nucleic Acids Research. 1994 ; Vol. 22, No. 18. pp. 3779-3786.
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