Organotypic cultures as tools for optimizing central nervous system cell therapies

Nicolas Daviaud, Elisa Garbayo, Paul C Schiller, Miguel Perez-Pinzon, Claudia N. Montero-Menei

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Stem cell therapy is a promising treatment for neurological disorders such as cerebral ischemia, Parkinson's disease and Huntington's disease. In recent years, many clinical trials with various cell types have been performed often showing mixed results. Major problems with cell therapies are the limited cell availability and engraftment and the reduced integration of grafted cells into the host tissue. Stem cell-based therapies can provide a limitless source of cells but survival and differentiation remain a drawback. An improved understanding of the behaviour of stem cells and their interaction with the host tissue, upon implantation, is needed to maximize the therapeutic potential of stem cells in neurological disorders. Organotypic cultures made from brain slices from specific brain regions that can be kept in culture for several weeks after injecting molecules or cells represent a remarkable tool to address these issues. This model allows the researcher to monitor/assess the behaviour and responses of both the endogenous as well as the implanted cells and their interaction with the microenvironment leading to cell engraftment. Moreover, organotypic cultures could be useful to partially model the pathological state of a disease in the brain and to study graft-host interactions prior to testing such grafts for pre-clinical applications. Finally, they can be used to test the therapeutic potential of stem cells when combined with scaffolds, or other therapeutic enhancers, among other aspects, needed to develop novel successful therapeutic strategies or improve on existing ones.

Original languageEnglish
Pages (from-to)429-440
Number of pages12
JournalExperimental Neurology
Volume248
DOIs
StatePublished - Oct 1 2013

Fingerprint

Cell- and Tissue-Based Therapy
Stem Cells
Central Nervous System
Nervous System Diseases
Cell Communication
Transplants
Therapeutics
Huntington Disease
Brain
Brain Diseases
Brain Ischemia
Parkinson Disease
Cell Differentiation
Cell Survival
Research Personnel
Clinical Trials

Keywords

  • Cell behaviour
  • Cell therapy
  • Neurodegenerative disorders
  • Organotypic slices
  • Stem cells
  • Tissue engineering

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience

Cite this

Organotypic cultures as tools for optimizing central nervous system cell therapies. / Daviaud, Nicolas; Garbayo, Elisa; Schiller, Paul C; Perez-Pinzon, Miguel; Montero-Menei, Claudia N.

In: Experimental Neurology, Vol. 248, 01.10.2013, p. 429-440.

Research output: Contribution to journalArticle

Daviaud, Nicolas ; Garbayo, Elisa ; Schiller, Paul C ; Perez-Pinzon, Miguel ; Montero-Menei, Claudia N. / Organotypic cultures as tools for optimizing central nervous system cell therapies. In: Experimental Neurology. 2013 ; Vol. 248. pp. 429-440.
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