Organizational differences in the membrane proteins of normal and irreversibly sickled erythrocytes

Robert W. Rubin; Clara Milikowski; Gary E. Wise

doi:10.1016/0005-2736(80)90241-2

Organizational differences in the membrane proteins of normal and irreversibly sickled erythrocytes

Robert W. Rubin, Clara Milikowski, Gary E. Wise

Pathology

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Using two-dimensional gels, no unique membrane proteins were detected in irreversibly sickled cells. Membranes from irreversibly sickled cells were shown to cross-link much more readily with dithiobis(succinimidyl propionate) than normal erythrocyte membranes. Increased binding of band 4.5 protein and increased intra-chain disulfides were also demonstrated. These changes may correlate to enhanced cellular rigidity.

Original language	English (US)
Pages (from-to)	1-8
Number of pages	8
Journal	BBA - Biomembranes
Volume	595
Issue number	1
DOIs	https://doi.org/10.1016/0005-2736(80)90241-2
State	Published - Jan 12 1980

Keywords

(Lymphocyte)
Capping
Fluorescent probe
Ion flux
Potential change

ASJC Scopus subject areas

Biochemistry
Biophysics
Cell Biology
Medicine(all)

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abstract = "Using two-dimensional gels, no unique membrane proteins were detected in irreversibly sickled cells. Membranes from irreversibly sickled cells were shown to cross-link much more readily with dithiobis(succinimidyl propionate) than normal erythrocyte membranes. Increased binding of band 4.5 protein and increased intra-chain disulfides were also demonstrated. These changes may correlate to enhanced cellular rigidity.",

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AU - Rubin, Robert W.

AU - Milikowski, Clara

AU - Wise, Gary E.

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N2 - Using two-dimensional gels, no unique membrane proteins were detected in irreversibly sickled cells. Membranes from irreversibly sickled cells were shown to cross-link much more readily with dithiobis(succinimidyl propionate) than normal erythrocyte membranes. Increased binding of band 4.5 protein and increased intra-chain disulfides were also demonstrated. These changes may correlate to enhanced cellular rigidity.

AB - Using two-dimensional gels, no unique membrane proteins were detected in irreversibly sickled cells. Membranes from irreversibly sickled cells were shown to cross-link much more readily with dithiobis(succinimidyl propionate) than normal erythrocyte membranes. Increased binding of band 4.5 protein and increased intra-chain disulfides were also demonstrated. These changes may correlate to enhanced cellular rigidity.

KW - (Lymphocyte)

KW - Capping

KW - Fluorescent probe

KW - Ion flux

KW - Potential change

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