Abstract
Using two-dimensional gels, no unique membrane proteins were detected in irreversibly sickled cells. Membranes from irreversibly sickled cells were shown to cross-link much more readily with dithiobis(succinimidyl propionate) than normal erythrocyte membranes. Increased binding of band 4.5 protein and increased intra-chain disulfides were also demonstrated. These changes may correlate to enhanced cellular rigidity.
| Original language | English |
|---|---|
| Pages (from-to) | 1-8 |
| Number of pages | 8 |
| Journal | BBA - Biomembranes |
| Volume | 595 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 12 1980 |
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Keywords
- (Lymphocyte)
- Capping
- Fluorescent probe
- Ion flux
- Potential change
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Cell Biology
- Medicine(all)
Cite this
Organizational differences in the membrane proteins of normal and irreversibly sickled erythrocytes. / Rubin, Robert W.; Milikowski, Clara; Wise, Gary E.
In: BBA - Biomembranes, Vol. 595, No. 1, 12.01.1980, p. 1-8.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Organizational differences in the membrane proteins of normal and irreversibly sickled erythrocytes
AU - Rubin, Robert W.
AU - Milikowski, Clara
AU - Wise, Gary E.
PY - 1980/1/12
Y1 - 1980/1/12
N2 - Using two-dimensional gels, no unique membrane proteins were detected in irreversibly sickled cells. Membranes from irreversibly sickled cells were shown to cross-link much more readily with dithiobis(succinimidyl propionate) than normal erythrocyte membranes. Increased binding of band 4.5 protein and increased intra-chain disulfides were also demonstrated. These changes may correlate to enhanced cellular rigidity.
AB - Using two-dimensional gels, no unique membrane proteins were detected in irreversibly sickled cells. Membranes from irreversibly sickled cells were shown to cross-link much more readily with dithiobis(succinimidyl propionate) than normal erythrocyte membranes. Increased binding of band 4.5 protein and increased intra-chain disulfides were also demonstrated. These changes may correlate to enhanced cellular rigidity.
KW - (Lymphocyte)
KW - Capping
KW - Fluorescent probe
KW - Ion flux
KW - Potential change
UR - http://www.scopus.com/inward/record.url?scp=0018898502&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0018898502&partnerID=8YFLogxK
U2 - 10.1016/0005-2736(80)90241-2
DO - 10.1016/0005-2736(80)90241-2
M3 - Article
C2 - 7349873
AN - SCOPUS:0018898502
VL - 595
SP - 1
EP - 8
JO - Biochimica et Biophysica Acta - Biomembranes
JF - Biochimica et Biophysica Acta - Biomembranes
SN - 0005-2736
IS - 1
ER -