Abstract
The galactose operon encoding a repressor and genes for the Leloir pathway for galactose metabolism (galactokinase, galactose-1-phosphate-uridyl transferase and UDP glucose-4-epimerase) was located adjacent to the multiple sugar metabolism (msm) operon on the chromosome of Streptococcus mutans Ingbritt (serotype c) and the complete nucleotide sequence of this 5-kilobase region was determined. The Leloir pathway was induced by the presence of galactose in the growth medium or following the release of intracellular galactose after uptake and cleavage of α-galactosides by the multiple sugar metabolism system. Analysis of the mechanism of galactose transport confirmed the absence of a galactose-specific phosphotransferase system and suggested the presence of an inducible galactose permease. Evidence is presented that galactose transport is independent of the proton motive force and may be ATP-dependent.
| Original language | English |
|---|---|
| Pages (from-to) | 137-144 |
| Number of pages | 8 |
| Journal | Gene |
| Volume | 180 |
| Issue number | 1-2 |
| DOIs | |
| State | Published - Nov 21 1996 |
| Externally published | Yes |
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Keywords
- Catabolism
- Galactose
- Pathogenic bacteria
- Permease
- Promoter
- Repressor
- Streptococcus mutans
- Sugar transport
ASJC Scopus subject areas
- Genetics
Cite this
Organization and nucleotide sequence of the Streptococcus mutans galactose operon. / Ajdic, Dragana; Sutcliffe, Iain C.; Russell, Roy R B; Ferretti, Joseph J.
In: Gene, Vol. 180, No. 1-2, 21.11.1996, p. 137-144.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Organization and nucleotide sequence of the Streptococcus mutans galactose operon
AU - Ajdic, Dragana
AU - Sutcliffe, Iain C.
AU - Russell, Roy R B
AU - Ferretti, Joseph J.
PY - 1996/11/21
Y1 - 1996/11/21
N2 - The galactose operon encoding a repressor and genes for the Leloir pathway for galactose metabolism (galactokinase, galactose-1-phosphate-uridyl transferase and UDP glucose-4-epimerase) was located adjacent to the multiple sugar metabolism (msm) operon on the chromosome of Streptococcus mutans Ingbritt (serotype c) and the complete nucleotide sequence of this 5-kilobase region was determined. The Leloir pathway was induced by the presence of galactose in the growth medium or following the release of intracellular galactose after uptake and cleavage of α-galactosides by the multiple sugar metabolism system. Analysis of the mechanism of galactose transport confirmed the absence of a galactose-specific phosphotransferase system and suggested the presence of an inducible galactose permease. Evidence is presented that galactose transport is independent of the proton motive force and may be ATP-dependent.
AB - The galactose operon encoding a repressor and genes for the Leloir pathway for galactose metabolism (galactokinase, galactose-1-phosphate-uridyl transferase and UDP glucose-4-epimerase) was located adjacent to the multiple sugar metabolism (msm) operon on the chromosome of Streptococcus mutans Ingbritt (serotype c) and the complete nucleotide sequence of this 5-kilobase region was determined. The Leloir pathway was induced by the presence of galactose in the growth medium or following the release of intracellular galactose after uptake and cleavage of α-galactosides by the multiple sugar metabolism system. Analysis of the mechanism of galactose transport confirmed the absence of a galactose-specific phosphotransferase system and suggested the presence of an inducible galactose permease. Evidence is presented that galactose transport is independent of the proton motive force and may be ATP-dependent.
KW - Catabolism
KW - Galactose
KW - Pathogenic bacteria
KW - Permease
KW - Promoter
KW - Repressor
KW - Streptococcus mutans
KW - Sugar transport
UR - http://www.scopus.com/inward/record.url?scp=0030597367&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030597367&partnerID=8YFLogxK
U2 - 10.1016/S0378-1119(96)00434-9
DO - 10.1016/S0378-1119(96)00434-9
M3 - Article
C2 - 8973358
AN - SCOPUS:0030597367
VL - 180
SP - 137
EP - 144
JO - Gene
JF - Gene
SN - 0378-1119
IS - 1-2
ER -