Organization and nucleotide sequence of the Streptococcus mutans galactose operon

Dragana Ajdić, Iain C. Sutcliffe, Roy R.B. Russell, Joseph J. Ferretti

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

The galactose operon encoding a repressor and genes for the Leloir pathway for galactose metabolism (galactokinase, galactose-1-phosphate-uridyl transferase and UDP glucose-4-epimerase) was located adjacent to the multiple sugar metabolism (msm) operon on the chromosome of Streptococcus mutans Ingbritt (serotype c) and the complete nucleotide sequence of this 5-kilobase region was determined. The Leloir pathway was induced by the presence of galactose in the growth medium or following the release of intracellular galactose after uptake and cleavage of α-galactosides by the multiple sugar metabolism system. Analysis of the mechanism of galactose transport confirmed the absence of a galactose-specific phosphotransferase system and suggested the presence of an inducible galactose permease. Evidence is presented that galactose transport is independent of the proton motive force and may be ATP-dependent.

Original languageEnglish (US)
Pages (from-to)137-144
Number of pages8
JournalGene
Volume180
Issue number1-2
DOIs
StatePublished - Nov 21 1996

    Fingerprint

Keywords

  • Catabolism
  • Galactose
  • Pathogenic bacteria
  • Permease
  • Promoter
  • Repressor
  • Streptococcus mutans
  • Sugar transport

ASJC Scopus subject areas

  • Genetics

Cite this