Organization and nucleotide sequence of the Streptococcus mutans galactose operon

Dragana Ajdic, Iain C. Sutcliffe, Roy R B Russell, Joseph J. Ferretti

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The galactose operon encoding a repressor and genes for the Leloir pathway for galactose metabolism (galactokinase, galactose-1-phosphate-uridyl transferase and UDP glucose-4-epimerase) was located adjacent to the multiple sugar metabolism (msm) operon on the chromosome of Streptococcus mutans Ingbritt (serotype c) and the complete nucleotide sequence of this 5-kilobase region was determined. The Leloir pathway was induced by the presence of galactose in the growth medium or following the release of intracellular galactose after uptake and cleavage of α-galactosides by the multiple sugar metabolism system. Analysis of the mechanism of galactose transport confirmed the absence of a galactose-specific phosphotransferase system and suggested the presence of an inducible galactose permease. Evidence is presented that galactose transport is independent of the proton motive force and may be ATP-dependent.

Original languageEnglish
Pages (from-to)137-144
Number of pages8
JournalGene
Volume180
Issue number1-2
DOIs
StatePublished - Nov 21 1996
Externally publishedYes

Fingerprint

Streptococcus mutans
Operon
Galactose
UDPglucose-Hexose-1-Phosphate Uridylyltransferase
UDPglucose 4-Epimerase
Galactokinase
Galactosides
Proton-Motive Force
Phosphotransferases
Chromosomes
Adenosine Triphosphate
Growth
Genes

Keywords

  • Catabolism
  • Galactose
  • Pathogenic bacteria
  • Permease
  • Promoter
  • Repressor
  • Streptococcus mutans
  • Sugar transport

ASJC Scopus subject areas

  • Genetics

Cite this

Organization and nucleotide sequence of the Streptococcus mutans galactose operon. / Ajdic, Dragana; Sutcliffe, Iain C.; Russell, Roy R B; Ferretti, Joseph J.

In: Gene, Vol. 180, No. 1-2, 21.11.1996, p. 137-144.

Research output: Contribution to journalArticle

Ajdic, Dragana ; Sutcliffe, Iain C. ; Russell, Roy R B ; Ferretti, Joseph J. / Organization and nucleotide sequence of the Streptococcus mutans galactose operon. In: Gene. 1996 ; Vol. 180, No. 1-2. pp. 137-144.
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