Organ-specific regulation of pro-inflammatory molecules in heart, lung, and kidney following brain death

Christian A. Skrabal, Larry O. Thompson, Evgenji V. Potapov, Robert E. Southard, David L. Joyce, Keith A. Youker, George P. Noon, Matthias Loebe

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Nonspecific inflammatory events following brain death may increase the intensity of the immunological host response. The present study investigated the course of pro-inflammatory molecules in heart, lung, kidney, and plasma after brain death induction. Brain death was induced in five pigs by inflation of an intracranial Foley catheter and five pigs were sham-operated as controls. Each experiment was terminated 6 h after brain death/sham operation and the organs were harvested. We measured the mRNA and protein levels for TNF-α, IL-1β, and IL-6 in heart, lung, kidney, and plasma. Additionally, the mRNA expression for IL-6R, ICAM-1, MCP-1, and TGF-β was determined in each organ. After 6 h, the plasma cytokine levels were higher in the brain-dead animals than in the sham-operated. In heart, lung, and kidney there was an increase in IL-6 and IL-1β following brain death, while TNF-α was up-regulated in lung only (P < 0.05). MCP-1 and TGF-β were significantly higher in heart and lung and IL-6R increased in heart after brain death (P < 0.05). Brain death was associated with non-uniform cytokine expression patterns in the investigated organs. These expression patterns may cause variable pro-inflammatory priming resulting in different degrees of damage and explain the organ-specific variation in outcomes after transplantations.

Original languageEnglish (US)
Pages (from-to)118-125
Number of pages8
JournalJournal of Surgical Research
Volume123
Issue number1
DOIs
StatePublished - Jan 2005
Externally publishedYes

Fingerprint

Brain Death
Kidney
Lung
Interleukin-1
Interleukin-6
Swine
Cytokines
Messenger RNA
Economic Inflation
Intercellular Adhesion Molecule-1
Catheters
Transplantation

Keywords

  • adhesion molecules
  • brain death
  • chemoattractant molecules
  • cytokines
  • donor organs
  • inflammation

ASJC Scopus subject areas

  • Surgery

Cite this

Organ-specific regulation of pro-inflammatory molecules in heart, lung, and kidney following brain death. / Skrabal, Christian A.; Thompson, Larry O.; Potapov, Evgenji V.; Southard, Robert E.; Joyce, David L.; Youker, Keith A.; Noon, George P.; Loebe, Matthias.

In: Journal of Surgical Research, Vol. 123, No. 1, 01.2005, p. 118-125.

Research output: Contribution to journalArticle

Skrabal, CA, Thompson, LO, Potapov, EV, Southard, RE, Joyce, DL, Youker, KA, Noon, GP & Loebe, M 2005, 'Organ-specific regulation of pro-inflammatory molecules in heart, lung, and kidney following brain death', Journal of Surgical Research, vol. 123, no. 1, pp. 118-125. https://doi.org/10.1016/j.jss.2004.07.245
Skrabal, Christian A. ; Thompson, Larry O. ; Potapov, Evgenji V. ; Southard, Robert E. ; Joyce, David L. ; Youker, Keith A. ; Noon, George P. ; Loebe, Matthias. / Organ-specific regulation of pro-inflammatory molecules in heart, lung, and kidney following brain death. In: Journal of Surgical Research. 2005 ; Vol. 123, No. 1. pp. 118-125.
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