ORF phage display to identify cellular proteins with different functions

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Open reading frame (ORF) phage display is a new branch of phage display aimed at improving its efficiency to identify cellular proteins with specific binding or functional activities. Despite the success of phage display with antibody libraries and random peptide libraries, phage display with cDNA libraries of cellular proteins identifies a high percentage of non-ORF clones encoding unnatural short peptides with minimal biological implications. This is mainly because of the uncontrollable reading frames of cellular proteins in conventional cDNA libraries. ORF phage display solves this problem by eliminating non-ORF clones to generate ORF cDNA libraries. Here I summarize the procedures of ORF phage display, discuss the factors influencing its efficiency, present examples of its versatile applications, and highlight evidence of its capability of identifying biologically relevant cellular proteins. ORF phage display coupled with different selection strategies is capable of delineating diverse functions of cellular proteins with unique advantages.

Original languageEnglish
Pages (from-to)2-9
Number of pages8
JournalMethods
Volume58
Issue number1
DOIs
StatePublished - Sep 1 2012

Fingerprint

Bacteriophages
Open Reading Frames
Display devices
Reading Frames
Gene Library
Proteins
Clone Cells
Peptide Library
Peptides
Antibodies

Keywords

  • Functional proteomics
  • Open reading frame phage display
  • ORF phage display
  • Phagocytosis ligand
  • Protein-protein interaction

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

ORF phage display to identify cellular proteins with different functions. / Li, Wei.

In: Methods, Vol. 58, No. 1, 01.09.2012, p. 2-9.

Research output: Contribution to journalArticle

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