Orbital pseudotumor can be a localized form of granulomatosis with polyangiitis as revealed by gene expression profiling

James T. Rosenbaum, Dongseok Choi, David J. Wilson, Hans E. Grossniklaus, Christina A. Harrington, Cailin H. Sibley, Roger A. Dailey, John D. Ng, Eric A. Steele, Craig N. Czyz, Jill A. Foster, David Tse, Chris Alabiad, Sander Dubovy, Prashant K. Parekh, Gerald J. Harris, Michael Kazim, Payal J. Patel, Valerie A. White, Peter J. DolmanBobby S. Korn, Don O. Kikkawa, Deepak P. Edward, Hind M. Alkatan, Hailah al-Hussain, R. Patrick Yeatts, Dinesh Selva, Patrick Stauffer, Stephen R. Planck

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Biopsies and ANCA testing for limited forms of granulomatosis with polyangiitis (GPA) are frequently non-diagnostic. We characterized gene expression in GPA and other causes of orbital inflammation. We tested the hypothesis that a sub-set of patients with non-specific orbital inflammation (NSOI, also known as pseudotumor) mimics a limited form of GPA. Formalin-fixed, paraffin-embedded orbital biopsies were obtained from controls (n. =. 20) and patients with GPA (n. =. 6), NSOI (n. =. 25), sarcoidosis (n. =. 7), or thyroid eye disease (TED) (n. =. 20) and were divided into discovery and validation sets. Transcripts in the tissues were quantified using Affymetrix U133 Plus 2.0 microarrays.Distinct gene expression profiles for controls and subjects with GPA, TED, or sarcoidosis were evident by principal coordinate analyses. Compared with healthy controls, 285 probe sets had elevated signals in subjects with GPA and 1472 were decreased (>. 1.5-fold difference, false discovery rate adjusted p. <. 0.05). The immunoglobulin family of genes had the most dramatic increase in expression. Although gene expression in GPA could be readily distinguished from gene expression in TED, sarcoidosis, or controls, a comparison of gene expression in GPA versus NSOI found no statistically significant differences.Thus, forms of orbital inflammation can be distinguished based on gene expression. NSOI/pseudotumor is heterogeneous but often may be an unrecognized, localized form of GPA.

Original languageEnglish (US)
Pages (from-to)271-278
Number of pages8
JournalExperimental and Molecular Pathology
Volume99
Issue number2
DOIs
StatePublished - Oct 1 2015

Keywords

  • Gene expression profiling
  • Granulomatosis with polyangiitis
  • Microarray analysis
  • Molecular pathology
  • Orbit pathology
  • Orbital pseudotumor

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Clinical Biochemistry

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  • Cite this

    Rosenbaum, J. T., Choi, D., Wilson, D. J., Grossniklaus, H. E., Harrington, C. A., Sibley, C. H., Dailey, R. A., Ng, J. D., Steele, E. A., Czyz, C. N., Foster, J. A., Tse, D., Alabiad, C., Dubovy, S., Parekh, P. K., Harris, G. J., Kazim, M., Patel, P. J., White, V. A., ... Planck, S. R. (2015). Orbital pseudotumor can be a localized form of granulomatosis with polyangiitis as revealed by gene expression profiling. Experimental and Molecular Pathology, 99(2), 271-278. https://doi.org/10.1016/j.yexmp.2015.07.002