Optimization of transgene expression in sugar-cane cells

Joel González-Cabrera, Alberto Coego-González, Ariel F. Martínez-Gil, Gustavo A. De La Riva, Roberto I. Vázquez-Padrón

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Sugar-cane cells were transformed by electropotation using different genetic constructs to analyze the influence of the octopine synthetase gene enhancer (ocs) and the first intron-exon from rice act1 gene (a-E/l/E), upon gusA transgene expression. The quadruplicated ocs enhancer (4ocs) increased 6-fold the expression of the reporter gene. The introduction of the a-E/l/E into the transcriptional unit of the 4ocs/35S::gusA chimeric gene increased β-glucuronidase (Gus) activity more than 69 times in transformed sugar-cane cells. The lack of the 5'end of the a-E/l/E sequence led to a drastic decrease in the expression levels of the reporter gene.

Original languageEnglish (US)
Pages (from-to)793-796
Number of pages4
JournalBiotechnology Techniques
Volume12
Issue number10
DOIs
StatePublished - Dec 9 1998
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Applied Microbiology and Biotechnology

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