Sugar-cane cells were transformed by electropotation using different genetic constructs to analyze the influence of the octopine synthetase gene enhancer (ocs) and the first intron-exon from rice act1 gene (a-E/l/E), upon gusA transgene expression. The quadruplicated ocs enhancer (4ocs) increased 6-fold the expression of the reporter gene. The introduction of the a-E/l/E into the transcriptional unit of the 4ocs/35S::gusA chimeric gene increased β-glucuronidase (Gus) activity more than 69 times in transformed sugar-cane cells. The lack of the 5'end of the a-E/l/E sequence led to a drastic decrease in the expression levels of the reporter gene.
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology