Abstract
Sugar-cane cells were transformed by electropotation using different genetic constructs to analyze the influence of the octopine synthetase gene enhancer (ocs) and the first intron-exon from rice act1 gene (a-E/l/E), upon gusA transgene expression. The quadruplicated ocs enhancer (4ocs) increased 6-fold the expression of the reporter gene. The introduction of the a-E/l/E into the transcriptional unit of the 4ocs/35S::gusA chimeric gene increased β-glucuronidase (Gus) activity more than 69 times in transformed sugar-cane cells. The lack of the 5'end of the a-E/l/E sequence led to a drastic decrease in the expression levels of the reporter gene.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 793-796 |
| Number of pages | 4 |
| Journal | Biotechnology Techniques |
| Volume | 12 |
| Issue number | 10 |
| DOIs | |
| State | Published - Dec 9 1998 |
| Externally published | Yes |
ASJC Scopus subject areas
- Biochemistry
- Applied Microbiology and Biotechnology
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Cite this
González-Cabrera, J., Coego-González, A., Martínez-Gil, A. F., De La Riva, G. A., & Vázquez-Padrón, R. I. (1998). Optimization of transgene expression in sugar-cane cells. Biotechnology Techniques, 12(10), 793-796. https://doi.org/10.1023/A:1008804432204