Optimization of transgene expression in sugar-cane cells

Joel González-Cabrera, Alberto Coego-González, Ariel F. Martínez-Gil, Gustavo A. De La Riva, Roberto I Vazquez-Padron

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Sugar-cane cells were transformed by electropotation using different genetic constructs to analyze the influence of the octopine synthetase gene enhancer (ocs) and the first intron-exon from rice act1 gene (a-E/l/E), upon gusA transgene expression. The quadruplicated ocs enhancer (4ocs) increased 6-fold the expression of the reporter gene. The introduction of the a-E/l/E into the transcriptional unit of the 4ocs/35S::gusA chimeric gene increased β-glucuronidase (Gus) activity more than 69 times in transformed sugar-cane cells. The lack of the 5'end of the a-E/l/E sequence led to a drastic decrease in the expression levels of the reporter gene.

Original languageEnglish
Pages (from-to)793-796
Number of pages4
JournalBiotechnology Techniques
Volume12
Issue number10
DOIs
StatePublished - Dec 9 1998
Externally publishedYes

Fingerprint

Sugar cane
Canes
Transgenes
Genes
Reporter Genes
Glucuronidase
Ligases
Introns
Exons

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Biochemistry

Cite this

González-Cabrera, J., Coego-González, A., Martínez-Gil, A. F., De La Riva, G. A., & Vazquez-Padron, R. I. (1998). Optimization of transgene expression in sugar-cane cells. Biotechnology Techniques, 12(10), 793-796. https://doi.org/10.1023/A:1008804432204

Optimization of transgene expression in sugar-cane cells. / González-Cabrera, Joel; Coego-González, Alberto; Martínez-Gil, Ariel F.; De La Riva, Gustavo A.; Vazquez-Padron, Roberto I.

In: Biotechnology Techniques, Vol. 12, No. 10, 09.12.1998, p. 793-796.

Research output: Contribution to journalArticle

González-Cabrera, J, Coego-González, A, Martínez-Gil, AF, De La Riva, GA & Vazquez-Padron, RI 1998, 'Optimization of transgene expression in sugar-cane cells', Biotechnology Techniques, vol. 12, no. 10, pp. 793-796. https://doi.org/10.1023/A:1008804432204
González-Cabrera J, Coego-González A, Martínez-Gil AF, De La Riva GA, Vazquez-Padron RI. Optimization of transgene expression in sugar-cane cells. Biotechnology Techniques. 1998 Dec 9;12(10):793-796. https://doi.org/10.1023/A:1008804432204
González-Cabrera, Joel ; Coego-González, Alberto ; Martínez-Gil, Ariel F. ; De La Riva, Gustavo A. ; Vazquez-Padron, Roberto I. / Optimization of transgene expression in sugar-cane cells. In: Biotechnology Techniques. 1998 ; Vol. 12, No. 10. pp. 793-796.
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