Abstract
Sugar-cane cells were transformed by electropotation using different genetic constructs to analyze the influence of the octopine synthetase gene enhancer (ocs) and the first intron-exon from rice act1 gene (a-E/l/E), upon gusA transgene expression. The quadruplicated ocs enhancer (4ocs) increased 6-fold the expression of the reporter gene. The introduction of the a-E/l/E into the transcriptional unit of the 4ocs/35S::gusA chimeric gene increased β-glucuronidase (Gus) activity more than 69 times in transformed sugar-cane cells. The lack of the 5'end of the a-E/l/E sequence led to a drastic decrease in the expression levels of the reporter gene.
| Original language | English |
|---|---|
| Pages (from-to) | 793-796 |
| Number of pages | 4 |
| Journal | Biotechnology Techniques |
| Volume | 12 |
| Issue number | 10 |
| DOIs | |
| State | Published - Dec 9 1998 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
- Biochemistry
Cite this
Optimization of transgene expression in sugar-cane cells. / González-Cabrera, Joel; Coego-González, Alberto; Martínez-Gil, Ariel F.; De La Riva, Gustavo A.; Vazquez-Padron, Roberto I.
In: Biotechnology Techniques, Vol. 12, No. 10, 09.12.1998, p. 793-796.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Optimization of transgene expression in sugar-cane cells
AU - González-Cabrera, Joel
AU - Coego-González, Alberto
AU - Martínez-Gil, Ariel F.
AU - De La Riva, Gustavo A.
AU - Vazquez-Padron, Roberto I
PY - 1998/12/9
Y1 - 1998/12/9
N2 - Sugar-cane cells were transformed by electropotation using different genetic constructs to analyze the influence of the octopine synthetase gene enhancer (ocs) and the first intron-exon from rice act1 gene (a-E/l/E), upon gusA transgene expression. The quadruplicated ocs enhancer (4ocs) increased 6-fold the expression of the reporter gene. The introduction of the a-E/l/E into the transcriptional unit of the 4ocs/35S::gusA chimeric gene increased β-glucuronidase (Gus) activity more than 69 times in transformed sugar-cane cells. The lack of the 5'end of the a-E/l/E sequence led to a drastic decrease in the expression levels of the reporter gene.
AB - Sugar-cane cells were transformed by electropotation using different genetic constructs to analyze the influence of the octopine synthetase gene enhancer (ocs) and the first intron-exon from rice act1 gene (a-E/l/E), upon gusA transgene expression. The quadruplicated ocs enhancer (4ocs) increased 6-fold the expression of the reporter gene. The introduction of the a-E/l/E into the transcriptional unit of the 4ocs/35S::gusA chimeric gene increased β-glucuronidase (Gus) activity more than 69 times in transformed sugar-cane cells. The lack of the 5'end of the a-E/l/E sequence led to a drastic decrease in the expression levels of the reporter gene.
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UR - http://www.scopus.com/inward/citedby.url?scp=0031741937&partnerID=8YFLogxK
U2 - 10.1023/A:1008804432204
DO - 10.1023/A:1008804432204
M3 - Article
AN - SCOPUS:0031741937
VL - 12
SP - 793
EP - 796
JO - Biotechnology Letters
JF - Biotechnology Letters
SN - 0141-5492
IS - 10
ER -