Optimization of the sequence of antigen loading and CD40-ligand-induced maturation of dendritic cells

Michael A. Morse, H. Kim Lyerly, Eli Gilboa, Elaine Thomas, Smita K. Nair

Research output: Contribution to journalArticle

76 Scopus citations

Abstract

Dendritic cells (DCs), matured by CD40-ligand (CD40L), undergo marked changes in their ability to process and present antigen, resulting in augmented lymphocyte stimulatory activity. We demonstrate that the form of the tumor antigen (peptide or genetic material) used to load the DCs dictates the required sequence of antigen loading and maturation for antitumor immunotherapy. Optimal stimulation of carcinoembryonic antigen (CEA)-specific CTLs by peptide-loaded DCs occurs when DCs from cancer patients are matured with CD40L before exposure to CEA peptide, whereas optimal stimulation by RNA-transfected DCs occurs when the DCs are loaded with CEA RNA before maturation with CD40L.

Original languageEnglish (US)
Pages (from-to)2965-2968
Number of pages4
JournalCancer Research
Volume58
Issue number14
StatePublished - Jul 15 1998
Externally publishedYes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Morse, M. A., Lyerly, H. K., Gilboa, E., Thomas, E., & Nair, S. K. (1998). Optimization of the sequence of antigen loading and CD40-ligand-induced maturation of dendritic cells. Cancer Research, 58(14), 2965-2968.