Optimization of perfluorocarbon emulsions for cellular encapsulation

Christopher Fraker, C. L. Stabler

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This study examined the parameters governing the formation of stable oxygen carrying emulsions for use in cellular encapsulation. Select perfluorocarbon, surfactant and physiological salt solution mixtures were manufactured and compared. Dynamic light scattering (DLS) particle size analysis, Fourier transform infrared spectroscopy (FTIR), measurements of effective oxygen diffusivity and toxicity assessments in cell culture were utilized to generate optimized solutions for use in microencapsulation of cellular products. The most stable, non-toxic and oxygen permeable emulsions were made from low w/v percent pluronic polaxamers dissolved in physiological saline and emulsified with 10% v/v perfluorotributylamine (FC-43). These emulsions had stable particle sizes at room temperature, little or no toxicity in cell co-cultures with the surfactant solution and enhanced diffusivity relative to the physiological salt solution.

Original languageEnglish
Title of host publicationIFMBE Proceedings
Pages165-166
Number of pages2
Volume24
DOIs
StatePublished - Nov 6 2009
Event25th Southern Biomedical Engineering Conference 2009 - Miami, FL, United States
Duration: May 15 2009May 17 2009

Other

Other25th Southern Biomedical Engineering Conference 2009
CountryUnited States
CityMiami, FL
Period5/15/095/17/09

Fingerprint

Fluorocarbons
Emulsions
Encapsulation
Oxygen
Surface-Active Agents
Toxicity
Surface active agents
Salts
Microencapsulation
Poloxamer
Dynamic light scattering
Cell culture
Particle size analysis
Fourier transform infrared spectroscopy
Particle size
Temperature
perfluorotributylamine

Keywords

  • Emulsions
  • Encapsulation
  • Perfluorocarbons

ASJC Scopus subject areas

  • Biomedical Engineering
  • Bioengineering

Cite this

Optimization of perfluorocarbon emulsions for cellular encapsulation. / Fraker, Christopher; Stabler, C. L.

IFMBE Proceedings. Vol. 24 2009. p. 165-166.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Fraker, C & Stabler, CL 2009, Optimization of perfluorocarbon emulsions for cellular encapsulation. in IFMBE Proceedings. vol. 24, pp. 165-166, 25th Southern Biomedical Engineering Conference 2009, Miami, FL, United States, 5/15/09. https://doi.org/10.1007/978-3-642-01697-4_60
Fraker, Christopher ; Stabler, C. L. / Optimization of perfluorocarbon emulsions for cellular encapsulation. IFMBE Proceedings. Vol. 24 2009. pp. 165-166
@inproceedings{f60e48401e5c45339b61e4653c8c9cd1,
title = "Optimization of perfluorocarbon emulsions for cellular encapsulation",
abstract = "This study examined the parameters governing the formation of stable oxygen carrying emulsions for use in cellular encapsulation. Select perfluorocarbon, surfactant and physiological salt solution mixtures were manufactured and compared. Dynamic light scattering (DLS) particle size analysis, Fourier transform infrared spectroscopy (FTIR), measurements of effective oxygen diffusivity and toxicity assessments in cell culture were utilized to generate optimized solutions for use in microencapsulation of cellular products. The most stable, non-toxic and oxygen permeable emulsions were made from low w/v percent pluronic polaxamers dissolved in physiological saline and emulsified with 10{\%} v/v perfluorotributylamine (FC-43). These emulsions had stable particle sizes at room temperature, little or no toxicity in cell co-cultures with the surfactant solution and enhanced diffusivity relative to the physiological salt solution.",
keywords = "Emulsions, Encapsulation, Perfluorocarbons",
author = "Christopher Fraker and Stabler, {C. L.}",
year = "2009",
month = "11",
day = "6",
doi = "10.1007/978-3-642-01697-4_60",
language = "English",
isbn = "9783642016967",
volume = "24",
pages = "165--166",
booktitle = "IFMBE Proceedings",

}

TY - GEN

T1 - Optimization of perfluorocarbon emulsions for cellular encapsulation

AU - Fraker, Christopher

AU - Stabler, C. L.

PY - 2009/11/6

Y1 - 2009/11/6

N2 - This study examined the parameters governing the formation of stable oxygen carrying emulsions for use in cellular encapsulation. Select perfluorocarbon, surfactant and physiological salt solution mixtures were manufactured and compared. Dynamic light scattering (DLS) particle size analysis, Fourier transform infrared spectroscopy (FTIR), measurements of effective oxygen diffusivity and toxicity assessments in cell culture were utilized to generate optimized solutions for use in microencapsulation of cellular products. The most stable, non-toxic and oxygen permeable emulsions were made from low w/v percent pluronic polaxamers dissolved in physiological saline and emulsified with 10% v/v perfluorotributylamine (FC-43). These emulsions had stable particle sizes at room temperature, little or no toxicity in cell co-cultures with the surfactant solution and enhanced diffusivity relative to the physiological salt solution.

AB - This study examined the parameters governing the formation of stable oxygen carrying emulsions for use in cellular encapsulation. Select perfluorocarbon, surfactant and physiological salt solution mixtures were manufactured and compared. Dynamic light scattering (DLS) particle size analysis, Fourier transform infrared spectroscopy (FTIR), measurements of effective oxygen diffusivity and toxicity assessments in cell culture were utilized to generate optimized solutions for use in microencapsulation of cellular products. The most stable, non-toxic and oxygen permeable emulsions were made from low w/v percent pluronic polaxamers dissolved in physiological saline and emulsified with 10% v/v perfluorotributylamine (FC-43). These emulsions had stable particle sizes at room temperature, little or no toxicity in cell co-cultures with the surfactant solution and enhanced diffusivity relative to the physiological salt solution.

KW - Emulsions

KW - Encapsulation

KW - Perfluorocarbons

UR - http://www.scopus.com/inward/record.url?scp=70350604729&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70350604729&partnerID=8YFLogxK

U2 - 10.1007/978-3-642-01697-4_60

DO - 10.1007/978-3-642-01697-4_60

M3 - Conference contribution

SN - 9783642016967

VL - 24

SP - 165

EP - 166

BT - IFMBE Proceedings

ER -