Optimization of perfluorocarbon emulsions for cellular encapsulation

C. Fraker, Cherie Stabler

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This study examined the parameters governing the formation of stable oxygen carrying emulsions for use in cellular encapsulation. Select perfluorocarbon, surfactant and physiological salt solution mixtures were manufactured and compared. Dynamic light scattering (DLS) particle size analysis, Fourier transform infrared spectroscopy (FTIR), measurements of effective oxygen diffusivity and toxicity assessments in cell culture were utilized to generate optimized solutions for use in microencapsulation of cellular products. The most stable, non-toxic and oxygen permeable emulsions were made from low w/v percent pluronic polaxamers dissolved in physiological saline and emulsified with 10% v/v perfluorotributylamine (FC-43). These emulsions had stable particle sizes at room temperature, little or no toxicity in cell co-cultures with the surfactant solution and enhanced diffusivity relative to the physiological salt solution.

Original languageEnglish (US)
Title of host publication25th Southern Biomedical Engineering Conference 2009
Pages165-166
Number of pages2
DOIs
StatePublished - Nov 6 2009
Event25th Southern Biomedical Engineering Conference 2009 - Miami, FL, United States
Duration: May 15 2009May 17 2009

Publication series

NameIFMBE Proceedings
Volume24
ISSN (Print)1680-0737

Other

Other25th Southern Biomedical Engineering Conference 2009
CountryUnited States
CityMiami, FL
Period5/15/095/17/09

Keywords

  • Emulsions
  • Encapsulation
  • Perfluorocarbons

ASJC Scopus subject areas

  • Biomedical Engineering
  • Bioengineering

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