TY - JOUR
T1 - Optical methods for probing mitochondrial function in brain slices
AU - Sick, Thomas J.
AU - Perez-Pinzon, Miguel A.
N1 - Funding Information:
This work was supported in part by Public Health Services grants N514325, N532167 (T. J. Sick) and N534773 (M. A. Perez-Pinzon).
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1999/6
Y1 - 1999/6
N2 - Brain slice preparations have become useful tools for studying multiple facets of normal brain function and for investigations of brain pathophysiology. Recently, a variety of neurological disorders have been linked to dysfunction of brain mitochondria. In this report we discuss optical methods for probing mitochondrial function in brain slices. Absorption spectrophotometric and spectrofluorometric techniques are described for measuring changes in the redox activity of mitochondrial cytochromes and the primary respiratory chain substrate nicotinamide adenine dinucleotide (NADH), respectively. A spectrofluorometric method is described also for measuring changes in mitochondrial membrane potential using the potential-sensitive fluorescent indicator JC-1. These methods used together have proven to be useful for studying dysfunction of mitochondria following in vitro ischemia in hippocampal slices, and might also be valuable for investigations of mitochondrial involvement in other neurological disorders.
AB - Brain slice preparations have become useful tools for studying multiple facets of normal brain function and for investigations of brain pathophysiology. Recently, a variety of neurological disorders have been linked to dysfunction of brain mitochondria. In this report we discuss optical methods for probing mitochondrial function in brain slices. Absorption spectrophotometric and spectrofluorometric techniques are described for measuring changes in the redox activity of mitochondrial cytochromes and the primary respiratory chain substrate nicotinamide adenine dinucleotide (NADH), respectively. A spectrofluorometric method is described also for measuring changes in mitochondrial membrane potential using the potential-sensitive fluorescent indicator JC-1. These methods used together have proven to be useful for studying dysfunction of mitochondria following in vitro ischemia in hippocampal slices, and might also be valuable for investigations of mitochondrial involvement in other neurological disorders.
UR - http://www.scopus.com/inward/record.url?scp=0032828962&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032828962&partnerID=8YFLogxK
U2 - 10.1006/meth.1999.0763
DO - 10.1006/meth.1999.0763
M3 - Article
C2 - 10356340
AN - SCOPUS:0032828962
VL - 18
SP - 104
EP - 108
JO - Methods in Enzymology
JF - Methods in Enzymology
SN - 0076-6879
IS - 2
ER -