Optical manipulation of vesicles for optofluidic applications

A. E. Vasdekis, E. A. Scott, C. P. O'Neil, D. Psaltis, J. A. Hubbell

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this report, we review our recent results in the optical micromanipulation of vesicles. Traditionally, vesicle manipulation has been possible by employing photon momentum and optical trapping, giving rise to unique observations of vesicle shape changes and soft matter mechanics. Contrary to these attempts, we employ photon energy rather than momentum, by sensitizing vesicles with an oxidizing moiety. The later converts incident photons to reactive oxygen species, which in turn attack and compromise the stability of the vesicle membrane. Both coherent and incoherent radiation was employed. Polymersome re-organization into smaller diameter vesicles was possible by focusing the excitation beam in the vicinity of the polymersomes. Extended vesicle illumination with a collimated beam lead to their complete destabilization and micelle formation. Single particle analysis revealed that payload release takes place within seconds of illumination in an explosive burst. We will discuss the destabilization and payload release kinetics, as revealed by high resolution microscopy at the single particle level, as well as potential applications in single cell biomodulation.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
Volume8810
DOIs
StatePublished - Dec 11 2013
Externally publishedYes
EventOptical Trapping and Optical Micromanipulation X - San Diego, CA, United States
Duration: Aug 25 2013Aug 29 2013

Other

OtherOptical Trapping and Optical Micromanipulation X
CountryUnited States
CitySan Diego, CA
Period8/25/138/29/13

Fingerprint

Optofluidics
Vesicles
Manipulation
manipulators
Photons
destabilization
payloads
Momentum
photons
Lighting
illumination
beam leads
momentum
sensitizing
coherent radiation
Micelles
Photon
Particles (particulate matter)
attack
Reactive Oxygen Species

ASJC Scopus subject areas

  • Applied Mathematics
  • Computer Science Applications
  • Electrical and Electronic Engineering
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics

Cite this

Vasdekis, A. E., Scott, E. A., O'Neil, C. P., Psaltis, D., & Hubbell, J. A. (2013). Optical manipulation of vesicles for optofluidic applications. In Proceedings of SPIE - The International Society for Optical Engineering (Vol. 8810). [88101R] https://doi.org/10.1117/12.2026583

Optical manipulation of vesicles for optofluidic applications. / Vasdekis, A. E.; Scott, E. A.; O'Neil, C. P.; Psaltis, D.; Hubbell, J. A.

Proceedings of SPIE - The International Society for Optical Engineering. Vol. 8810 2013. 88101R.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Vasdekis, AE, Scott, EA, O'Neil, CP, Psaltis, D & Hubbell, JA 2013, Optical manipulation of vesicles for optofluidic applications. in Proceedings of SPIE - The International Society for Optical Engineering. vol. 8810, 88101R, Optical Trapping and Optical Micromanipulation X, San Diego, CA, United States, 8/25/13. https://doi.org/10.1117/12.2026583
Vasdekis AE, Scott EA, O'Neil CP, Psaltis D, Hubbell JA. Optical manipulation of vesicles for optofluidic applications. In Proceedings of SPIE - The International Society for Optical Engineering. Vol. 8810. 2013. 88101R https://doi.org/10.1117/12.2026583
Vasdekis, A. E. ; Scott, E. A. ; O'Neil, C. P. ; Psaltis, D. ; Hubbell, J. A. / Optical manipulation of vesicles for optofluidic applications. Proceedings of SPIE - The International Society for Optical Engineering. Vol. 8810 2013.
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