Optical coherence photoacoustic microscopy for in vivo multimodal retinal imaging

Xiaojing Liu, Tan Liu, Rong Wen, Yiwen Li, Carmen A. Puliafito, Hao F. Zhang, Shuliang Jiao

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

We developed an optical coherence photoacoustic microscopy (OC-PAM) system, which can accomplish optical coherence tomography (OCT) and photoacoustic microscopy (PAM) simultaneously by using a single pulsed broadband light source. With a center wavelength of 800 nm and a bandwidth of 30 nm, the system is suitable for imaging the retina. Generated from the same group of photons, the OCT and PAM images are intrinsically registered in the lateral directions. To test the capabilities of the system on multimodal ophthalmic imaging, we imaged the retina of pigmented rats. The OCT images showed the retinal structures with quality similar to conventional OCT, while the PAM images revealed the distribution of absorbers in the retina. Since the absorption of hemoglobin is relatively weak at around 800 nm, the NIR PAM signals are generated mainly from melanin in the posterior segment of the eye, thus providing melanin-specific imaging of the retina.

Original languageEnglish (US)
Pages (from-to)1370-1373
Number of pages4
JournalOptics Letters
Volume40
Issue number7
DOIs
StatePublished - 2015

Fingerprint

photoacoustic microscopy
retina
tomography
melanin
hemoglobin
rats
absorbers
light sources
broadband
bandwidth
photons
wavelengths

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Optical coherence photoacoustic microscopy for in vivo multimodal retinal imaging. / Liu, Xiaojing; Liu, Tan; Wen, Rong; Li, Yiwen; Puliafito, Carmen A.; Zhang, Hao F.; Jiao, Shuliang.

In: Optics Letters, Vol. 40, No. 7, 2015, p. 1370-1373.

Research output: Contribution to journalArticle

Liu, Xiaojing ; Liu, Tan ; Wen, Rong ; Li, Yiwen ; Puliafito, Carmen A. ; Zhang, Hao F. ; Jiao, Shuliang. / Optical coherence photoacoustic microscopy for in vivo multimodal retinal imaging. In: Optics Letters. 2015 ; Vol. 40, No. 7. pp. 1370-1373.
@article{281775eebb45458a8ce92d8fdd2369ec,
title = "Optical coherence photoacoustic microscopy for in vivo multimodal retinal imaging",
abstract = "We developed an optical coherence photoacoustic microscopy (OC-PAM) system, which can accomplish optical coherence tomography (OCT) and photoacoustic microscopy (PAM) simultaneously by using a single pulsed broadband light source. With a center wavelength of 800 nm and a bandwidth of 30 nm, the system is suitable for imaging the retina. Generated from the same group of photons, the OCT and PAM images are intrinsically registered in the lateral directions. To test the capabilities of the system on multimodal ophthalmic imaging, we imaged the retina of pigmented rats. The OCT images showed the retinal structures with quality similar to conventional OCT, while the PAM images revealed the distribution of absorbers in the retina. Since the absorption of hemoglobin is relatively weak at around 800 nm, the NIR PAM signals are generated mainly from melanin in the posterior segment of the eye, thus providing melanin-specific imaging of the retina.",
author = "Xiaojing Liu and Tan Liu and Rong Wen and Yiwen Li and Puliafito, {Carmen A.} and Zhang, {Hao F.} and Shuliang Jiao",
year = "2015",
doi = "10.1364/OL.40.001370",
language = "English (US)",
volume = "40",
pages = "1370--1373",
journal = "Optics Letters",
issn = "0146-9592",
publisher = "The Optical Society",
number = "7",

}

TY - JOUR

T1 - Optical coherence photoacoustic microscopy for in vivo multimodal retinal imaging

AU - Liu, Xiaojing

AU - Liu, Tan

AU - Wen, Rong

AU - Li, Yiwen

AU - Puliafito, Carmen A.

AU - Zhang, Hao F.

AU - Jiao, Shuliang

PY - 2015

Y1 - 2015

N2 - We developed an optical coherence photoacoustic microscopy (OC-PAM) system, which can accomplish optical coherence tomography (OCT) and photoacoustic microscopy (PAM) simultaneously by using a single pulsed broadband light source. With a center wavelength of 800 nm and a bandwidth of 30 nm, the system is suitable for imaging the retina. Generated from the same group of photons, the OCT and PAM images are intrinsically registered in the lateral directions. To test the capabilities of the system on multimodal ophthalmic imaging, we imaged the retina of pigmented rats. The OCT images showed the retinal structures with quality similar to conventional OCT, while the PAM images revealed the distribution of absorbers in the retina. Since the absorption of hemoglobin is relatively weak at around 800 nm, the NIR PAM signals are generated mainly from melanin in the posterior segment of the eye, thus providing melanin-specific imaging of the retina.

AB - We developed an optical coherence photoacoustic microscopy (OC-PAM) system, which can accomplish optical coherence tomography (OCT) and photoacoustic microscopy (PAM) simultaneously by using a single pulsed broadband light source. With a center wavelength of 800 nm and a bandwidth of 30 nm, the system is suitable for imaging the retina. Generated from the same group of photons, the OCT and PAM images are intrinsically registered in the lateral directions. To test the capabilities of the system on multimodal ophthalmic imaging, we imaged the retina of pigmented rats. The OCT images showed the retinal structures with quality similar to conventional OCT, while the PAM images revealed the distribution of absorbers in the retina. Since the absorption of hemoglobin is relatively weak at around 800 nm, the NIR PAM signals are generated mainly from melanin in the posterior segment of the eye, thus providing melanin-specific imaging of the retina.

UR - http://www.scopus.com/inward/record.url?scp=84944893505&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84944893505&partnerID=8YFLogxK

U2 - 10.1364/OL.40.001370

DO - 10.1364/OL.40.001370

M3 - Article

C2 - 25831335

AN - SCOPUS:84944893505

VL - 40

SP - 1370

EP - 1373

JO - Optics Letters

JF - Optics Letters

SN - 0146-9592

IS - 7

ER -