Optical ATP biosensor for extracellular ATP measurement

C. Wang, C. Y.C. Huang, W. C. Lin

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Extracellular Adenosine-5'-triphosphate (ATP) is an important multi-functional molecule which can mediate numerous physiological activities by activating purinergic P2 receptors. The objective of this study was to develop a novel optical ATP sensor for in-situ extracellular ATP measurement in biological tissues. The optical ATP sensor was made by applying two layers of sol-gel coating to the end of an optical fiber probe end. The first layer contained ruthenium complex for sensing changes in oxygen concentration which resulted from oxidation of ATP by glycerol kinase and glycerol 3-phosphate oxidase entrapped in the second layer. It was demonstrated that the optical ATP sensor was capable of detecting ATP concentration at a broad range of 10-3mM to 1.5mM.A compensation method was established to enable the optical sensor to determine ATP concentration at different oxygen levels. This study also demonstrated the capability of ATP sensor to measure extracellular ATP content in biological tissues (i.e., porcine intervertebral disc). In addition, it was shown that the optical ATP sensor was not affected by pH and derivatives of extracellular ATP. Therefore, the newly developed optical ATP sensor is a good option for in-situ extracellular ATP measurement.

Original languageEnglish (US)
Pages (from-to)355-361
Number of pages7
JournalBiosensors and Bioelectronics
Volume43
Issue number1
DOIs
StatePublished - May 5 2013

Keywords

  • ATP
  • Biosensor
  • Compensation methods
  • Optical
  • Ruthenium complex

ASJC Scopus subject areas

  • Biophysics
  • Biomedical Engineering
  • Biotechnology
  • Electrochemistry

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